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ZFIN ID: ZDB-FIG-151211-3
Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica   130(3):389-406 Full text @ Acta Neuropathol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 6

Fig. 4

Quantification of muscle function in Tg(ACTA1-eGFP) zebrafish. a Quantification of the maximum acceleration recorded from touch-evoked response assays of Tg(ACTA1D286G-eGFP)high and Tg(ACTA1wildtype-eGFP)high zebrafish compared to control zebrafish at 2 dpf. Error bars represent SD for n = 15–19 zebrafish, *p < 0.05. b qRT-PCR analysis of ACTA1-eGFP expression in transgenic lines at 2 dpf. No significant difference was observed between Tg(ACTA1D286G-eGFP)high and Tg(ACTA1wildtype-eGFP)high zebrafish. Error bars represent ±SEM for four replicate experiments with each experiment comprising a pooled samples of 20 fish, *p < 0.05, **p < 0.01. c, d Quantification of the c number of small movements and d distance traveled by Tg(ACTA1D286G-eGFP)high and Tg(ACTA1wildtype-eGFP)high and Tg(ACTA1D286G-eGFP)low and Tg(ACTA1wildtype-eGFP)low strains compared to control fish at 6 dpf. Error bars represent ±SEM for three replicate experiments (n = 48 per experiment), *p < 0.05, **p < 0.01

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mo1Tg; mo5Tg standard conditions Day 6 swimming decreased process quality, abnormal
mo2Tg; mo5Tg standard conditions Day 6 swimming decreased process quality, abnormal
mo3Tg; mo5Tg standard conditions Long-pec thigmotaxis decreased process quality, abnormal
Day 6 swimming decreased process quality, abnormal
mo4Tg; mo5Tg standard conditions Long-pec thigmotaxis decreased process quality, abnormal
Day 6 swimming decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Acta Neuropathologica for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Acta Neuropathol.