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ZFIN ID: ZDB-FIG-151019-11
Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica   130(3):389-406 Full text @ Acta Neuropathol.
Anatomical Terms:
Stage: Long-pec
Observed In:
Stage: Long-pec

Fig. 2

Characterization of skeletal muscle pathology in Tg(ACTA1D286G-eGFP)high zebrafish. a Skeletal muscle expressing i mosaic ACTA1D286G-eGFP, and ii overlaid with a light microscopy image of the same section showing Gomori trichrome staining, and iii enlarged. Dark regions (indicative of nemaline bodies) of disrupted muscle correspond to eGFP expression (arrows). b Correlative light and electron microscopy of Tg(ACTA1D286G-eGFP)high fish muscle at 2 dpf. b i Fluorescent image and corresponding ii electron microscopy image of skeletal muscle section containing a dense, elongated nemaline body (arrow) and enlarged in (iii). Sections are matched using nuclei positions (asterisk, plus and hash). c i Accumulations of actin filaments (arrowheads) and ii diffuse regions of filamentous actin (arrowheads), as well as ii disrupted sarcomeric regions are evident in Tg(ACTA1 D286G-eGFP)high skeletal muscle, at 2 dpf unlike the iii uniform sarcomeres observed in Tg(ACTA1-eGFP) zebrafish

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mo3Tg; mo5Tg standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mo3Tg; mo5Tg standard conditions Long-pec skeletal muscle structure, abnormal
Long-pec skeletal muscle actin filament accumulation skeletal muscle, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle filamentous actin distributed, abnormal
Long-pec skeletal muscle sarcomere disorganized, abnormal
Long-pec skeletal muscle sarcomere structure, abnormal
ZFIN wishes to thank the journal Acta Neuropathologica for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Acta Neuropathol.