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ZFIN ID: ZDB-FIG-151019-11
Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica   130(3):389-406 Full text @ Acta Neuropathol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 2

Characterization of skeletal muscle pathology in Tg(ACTA1D286G-eGFP)high zebrafish. a Skeletal muscle expressing i mosaic ACTA1D286G-eGFP, and ii overlaid with a light microscopy image of the same section showing Gomori trichrome staining, and iii enlarged. Dark regions (indicative of nemaline bodies) of disrupted muscle correspond to eGFP expression (arrows). b Correlative light and electron microscopy of Tg(ACTA1D286G-eGFP)high fish muscle at 2 dpf. b i Fluorescent image and corresponding ii electron microscopy image of skeletal muscle section containing a dense, elongated nemaline body (arrow) and enlarged in (iii). Sections are matched using nuclei positions (asterisk, plus and hash). c i Accumulations of actin filaments (arrowheads) and ii diffuse regions of filamentous actin (arrowheads), as well as ii disrupted sarcomeric regions are evident in Tg(ACTA1 D286G-eGFP)high skeletal muscle, at 2 dpf unlike the iii uniform sarcomeres observed in Tg(ACTA1-eGFP) zebrafish

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mo3Tg; mo5Tg standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mo3Tg; mo5Tg standard conditions Long-pec skeletal muscle structure, abnormal
Long-pec skeletal muscle actin filament accumulation skeletal muscle, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle filamentous actin distributed, abnormal
Long-pec skeletal muscle sarcomere disorganized, abnormal
Long-pec skeletal muscle sarcomere structure, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Acta Neuropathologica for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Acta Neuropathol.