header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-151019-12
Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica   130(3):389-406 Full text @ Acta Neuropathol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 3

Formation of nemaline bodies and aggregates in Tg(ACTA1 D286G-eGFP)high zebrafish. a Maximum projection images from time lapse of Tg(ACTA1D286G-eGFP)high fish from 56 to 71 hpf showing nemaline bodies distributed throughout the skeletal muscle (arrows). Nemaline bodies’ fragment from 59.5 hpf (arrows), coincident with the formation of aggregates at the myosepta (arrowheads). b Quantification of the mean number of nemaline bodies in Tg(ACTA1 D286G-eGFP) low (n = 50 per stage) and Tg(ACTA1 D286G-eGFP) (n = 48 per stage) strains. c Quantification of the percentage of fish displaying globular aggregates in Tg(ACTA1 D286G-eGFP) low and Tg(ACTA1 D286G-eGFP) high strains (n = 50 per stage). Error bars represent SEM from three independent experiments (n = 45 per replicate)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mo3Tg; mo5Tg standard conditions Long-pec myoseptum cellular anatomical entity IFL
Long-pec skeletal muscle cellular anatomical entity IFL
Pec-fin myoseptum cellular anatomical entity IFL
Pec-fin skeletal muscle cellular anatomical entity IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mo3Tg; mo5Tg standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
Pec-fin myoseptum cellular anatomical entity aggregated, abnormal
Pec-fin skeletal muscle cellular anatomical entity aggregated, abnormal
Protruding-mouth skeletal muscle cellular anatomical entity aggregated, abnormal
Day 4 skeletal muscle cellular anatomical entity aggregated, abnormal
Day 5 skeletal muscle cellular anatomical entity aggregated, abnormal
mo4Tg; mo5Tg standard conditions Protruding-mouth skeletal muscle cellular anatomical entity aggregated, abnormal
Day 4 skeletal muscle cellular anatomical entity aggregated, abnormal
Day 5 skeletal muscle cellular anatomical entity aggregated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Acta Neuropathologica for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Acta Neuropathol.