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ZFIN ID: ZDB-FIG-151019-16
Sztal et al., 2015 - Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function. Acta Neuropathologica   130(3):389-406 Full text @ Acta Neuropathol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 8

a Maximum projection confocal microscopy images of Tg(ACTA1wildtype-eGFP)low and Tg(ACTA1wildtype-eGFP)high zebrafish strains injected with two different Nebulin (Neb) morpholinos compared to control uninjected embryos at 2 dpf. There is an increased prevalence of eGFP-positive globular aggregates at the myosepta (arrowheads) in Tg(ACTA1wildtype-eGFP)high Neb morphants compared to controls. Knockdown of Neb produces globular aggregates at the myosepta (arrowheads) in Tg(ACTA1wildtype -eGFP)low Neb morphants that are absent in control uninjected embryos. b Maximum projection confocal microscopy images of wild-type embryos injected with Neb morpholinos at 2 dpf and stained with phalloidin shows an increase in actin-positive aggregates at the myosepta (arrowheads), which are absent in control uninjected embryos

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP mo1Tg; mo5Tg control Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo1Tg; mo5Tg + MO1-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo1Tg; mo5Tg + MO1-neb + MO2-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo1Tg; mo5Tg + MO2-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo2Tg; mo5Tg control Long-pec skeletal muscle IFL
mo2Tg; mo5Tg + MO1-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo2Tg; mo5Tg + MO1-neb + MO2-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
mo2Tg; mo5Tg + MO2-neb standard conditions Long-pec skeletal muscle IFL
Long-pec skeletal muscle cellular anatomical entity IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
mo1Tg; mo5Tg control Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo1Tg; mo5Tg + MO1-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo1Tg; mo5Tg + MO1-neb + MO2-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo1Tg; mo5Tg + MO2-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo2Tg; mo5Tg + MO1-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo2Tg; mo5Tg + MO1-neb + MO2-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
mo2Tg; mo5Tg + MO2-neb standard conditions Long-pec myoseptum cellular anatomical entity aggregated, abnormal
Long-pec skeletal muscle cellular anatomical entity aggregated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Acta Neuropathologica for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Acta Neuropathol.