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ZFIN ID: ZDB-FIG-151102-34
Ghaye et al., 2015 - Progenitor potential of nkx6.1-expressing cells throughout zebrafish life and during beta cell regeneration. BMC Biology   13:70 Full text @ BMC Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 7

Expression of nkx6.1 persists in duct cells in the adult pancreas. a GFP and insulin (Ins, red) immunodetection on section through the pancreas of adult Tg(nkx6.1:eGFP) zebrafish. White arrows point to pancreatic ducts and asterisks show cells (presumably centroacinar/ terminal end duct cells (CACs) dispersed throughout the exocrine tissue). a′ Close-up of the islet highlighted with Ins. b Venus (green) and Ins (red) immunodetection in Tg(Tp1:VenusPest) showing the presence of Venus in CACs (asterisks) as previously reported 12], but not in duct cells within ductular structures (white arrow). Insets in (a) and (b) show isolated CACs. cd Immunodetection of Venus (green) and of endogenous Nkx6.1 (red) in Tg(Tp1:VenusPest) revealing co-labeling of both markers in CACs (c, c′) while Nkx6.1 alone, but not Venus, labels the ducts. The white arrow in (d) points to a duct and asterisks indicate CACs. Dotted yellow lines delimit the duct. c′ Same as (c) showing Nkx6.1 (red) and 4′,6-diamidino-2-phenylindole (DAPI) only. i, islet

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP ulg004Tg(AB) standard conditions Adult pancreatic centroacinar cell IHC
Adult pancreatic duct IHC
Venus s940Tg standard conditions Adult pancreatic centroacinar cell IHC
Adult Not Detected pancreatic duct IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-nkx6 IHC s940Tg standard conditions Adult pancreatic centroacinar cell
IHC Adult pancreatic duct
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Biol.