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ZFIN ID: ZDB-FIG-151001-10
Yang et al., 2015 - Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development. Developmental Biology   404(2):49-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Conditions:
Anatomical Term:
Stage: Prim-15
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Prim-15

Fig. S1

VEGF and Notch regulate PLK2 expression in HUVECs and zebrafish vasculature. (A)RT-PCR and (B) Western analysis reveals that PLK2 mRNA and protein expression levels were elevated in HUVECs after VEGF treatment. (C) Quantitative measurements show that PLK2 mRNA (p=0.004) and protein level (p=0.015) in HUVECs is less after VEGF treatment. (D–G) Whole mount RNA in situ hybridization shows that (E, E′) VEGF pathway inhibition with SU5416 (n=29/33) treatment starting at 20 hpf resulted in decreased plk2b expression in the zebrafish vasculature at 32 hpf when compared to (D, D′) DMSO treatment (n=0/35), whereas (G, G′) Notch inhibition with dn-MAML overexpression (n=24/35) starting at 20 hpf resulted in increased plk2b expression in the zebrafish vasculature at 32 hpf when compared to the (F, F′) control siblings (n=0/35). D′–G′ images represent the boxed area in D–G. (H, I) Quantitative PCR confirms that plk2b expression level in sorted endothelial cells is decreased in (H) SU5416 treated fish (p=0.0178) and (I) increased in dn-MAML overexpression fish (p=0.0142). Mean±s.e.m. *p<0.05, **p<0.01 by Student′s t-test.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
plk2b WT control Prim-15 endothelial cell ISH
WT chemical treatment by environment: semaxanib Prim-15 endothelial cell ISH
fb14Tg heat shock Prim-15 endothelial cell ISH
fb14Tg ; s896Tg heat shock Prim-15 endothelial cell RTPCR
s896Tg chemical treatment by environment: semaxanib Prim-15 endothelial cell RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: semaxanib Prim-15 endothelial cell plk2b expression decreased amount, abnormal
fb14Tg heat shock Prim-15 endothelial cell plk2b expression increased amount, abnormal
fb14Tg ; s896Tg heat shock Prim-15 endothelial cell plk2b expression increased amount, abnormal
s896Tg chemical treatment by environment: semaxanib Prim-15 endothelial cell plk2b expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.