|ZFIN ID: ZDB-FIG-151001-10|
VEGF and Notch regulate PLK2 expression in HUVECs and zebrafish vasculature. (A)RT-PCR and (B) Western analysis reveals that PLK2 mRNA and protein expression levels were elevated in HUVECs after VEGF treatment. (C) Quantitative measurements show that PLK2 mRNA (p=0.004) and protein level (p=0.015) in HUVECs is less after VEGF treatment. (D–G) Whole mount RNA in situ hybridization shows that (E, E′) VEGF pathway inhibition with SU5416 (n=29/33) treatment starting at 20 hpf resulted in decreased plk2b expression in the zebrafish vasculature at 32 hpf when compared to (D, D′) DMSO treatment (n=0/35), whereas (G, G′) Notch inhibition with dn-MAML overexpression (n=24/35) starting at 20 hpf resulted in increased plk2b expression in the zebrafish vasculature at 32 hpf when compared to the (F, F′) control siblings (n=0/35). D′–G′ images represent the boxed area in D–G. (H, I) Quantitative PCR confirms that plk2b expression level in sorted endothelial cells is decreased in (H) SU5416 treated fish (p=0.0178) and (I) increased in dn-MAML overexpression fish (p=0.0142). Mean±s.e.m. *p<0.05, **p<0.01 by Student′s t-test.
|WT||chemical treatment by environment: semaxanib||Prim-15||endothelial cell||ISH|
|fb14Tg||heat shock||Prim-15||endothelial cell||ISH|
|fb14Tg ; s896Tg||heat shock||Prim-15||endothelial cell||RTPCR|
|s896Tg||chemical treatment by environment: semaxanib||Prim-15||endothelial cell||RTPCR|
|WT||chemical treatment by environment: semaxanib||Prim-15||endothelial cell plk2b expression decreased amount, abnormal|
|fb14Tg||heat shock||Prim-15||endothelial cell plk2b expression increased amount, abnormal|
|fb14Tg ; s896Tg||heat shock||Prim-15||endothelial cell plk2b expression increased amount, abnormal|
|s896Tg||chemical treatment by environment: semaxanib||Prim-15||endothelial cell plk2b expression decreased amount, abnormal|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
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Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.