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ZFIN ID: ZDB-FIG-151001-5
Yang et al., 2015 - Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development. Developmental Biology   404(2):49-60 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-25

Fig. 2

plk2b regulates endothelial cell sprouting. (A–D) Fluorescence micrographs show that loss of plk2b function in Tg(kdrl:mcherry-ras) zebrafish embryos results in underdeveloped intersomitic vessel sprouting (open arrowheads) at 36 hpf. (A) control MO (n=0/149); (B) plk2b ATG MO (MO1) (n=125/171); (C) plk2b splice MO (MO2) (n=89/137); (D) dn-plk2b RNA – dominant negative plk2b (n=49/85). Injecting (E) 80 pg of plk2b (n=31/103) or (F) 80 pg of hPLK2 (n=24/71) can rescue the plk2b MO1 vascular sprouting defect. (G) Injecting 80 pg of plk2b RNA into wild-type Tg(kdrl:mcherry-ras) embryos did not cause any significant vascular phenotypes (n=0/105). However, (H) injecting 160 pg of plk2b resulted in increased ISV spouting and branches (arrowheads) (n=91/145). Top, dorsal longitudinal anastomotic vessel (yellow asterisk); bottom, dorsal aorta/cardinal vein. Scale bar, 80 µm. Quantitative measurements of (I) intersomitic vessel length and (J) the number of ISV branches for each corresponding condition. Mean±s.e.m. *p<0.05 by ANOVA.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry s896Tg control Prim-25 intersegmental vessel IFL
Prim-25 trunk vasculature IFL
s896Tg + MO2-plk2b control Prim-25 intersegmental vessel IFL
Prim-25 trunk vasculature IFL
s896Tg + MO3-plk2b control Prim-25 intersegmental vessel IFL
Prim-25 trunk vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s896Tg + MO2-plk2b control Prim-25 dorsal longitudinal anastomotic vessel aplastic/hypoplastic, abnormal
s896Tg + MO2-plk2b control Prim-25 intersegmental vessel decreased length, abnormal
s896Tg + MO2-plk2b control Prim-25 intersegmental vessel hypoplastic, abnormal
s896Tg + MO2-plk2b control Prim-25 sprouting angiogenesis decreased occurrence, abnormal
s896Tg + MO3-plk2b control Prim-25 dorsal longitudinal anastomotic vessel aplastic/hypoplastic, abnormal
Prim-25 intersegmental vessel decreased length, abnormal
Prim-25 intersegmental vessel hypoplastic, abnormal
Prim-25 sprouting angiogenesis decreased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 404(2), Yang, H., Fang, L., Zhan, R., Hegarty, J.M., Ren, J., Hsiai, T.K., Gleeson, J.G., Miller, Y.I., Trejo, J., Chi, N.C., Polo-like kinase 2 regulates angiogenic sprouting and blood vessel development, 49-60, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.