ZFIN ID: ZDB-FIG-150928-12
Zhang et al., 2015 - Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish. PLoS One   10:e0132567 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 6

NAD(P)H signal is relatively stable during normal development but reduced in dystrophin morphants.

(A) NAD(P)H signal of white skeletal muscle cells of zebrafish embryo at 5dpf. Scale bar,10µm. (B) Intensity-based segmentation of mitochondria. NAD(P)H signal was defined as mean intensity of mitochondrial regions. (C) NAD(P)H autofluorescence in the mitochondria of red (right) and white (left) skeletal muscle cells of wild-type embryos at 2, 5 and 8 dpf. No significant change of NAD(P)H was observed during development in either red or white fibers. N = 5–13 animals per group. (D) NAD(P)H signal was significantly decreased in both red and white fibers of dys-MO morphants (dys-MO) compared with wild-type (WT) controls. Data are expressed as the mean±SEM. *: p<0.05; **: p<0.01 versus wild-type group.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TL + MO2-dmd standard conditions Day 5 fast muscle cell mitochondrial respirasome decreased functionality, abnormal
Day 5 slow muscle cell mitochondrial respirasome decreased functionality, abnormal
Day 5 slow muscle cell mitochondrion decreased functionality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One