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ZFIN ID: ZDB-PUB-150718-1
Remodeling of Mitochondrial Flashes in Muscular Development and Dystrophy in Zebrafish
Zhang, M., Sun, T., Jian, C., Lei, L., Han, P., Lv, Q., Yang, R., Zhou, X., Xu, J., Hu, Y., Men, Y., Huang, Y., Zhang, C., Zhu, X., Wang, X., Cheng, H., Xiong, J.W.
Date: 2015
Source: PLoS One   10: e0132567 (Journal)
Registered Authors: Han, Peidong, Xiong, Jing-Wei
Keywords: Mitochondria, Skeletal muscles, Embryos, Zebrafish, Skeletal muscle fibers, Dystrophin, Muscle fibers, Muscle cells
MeSH Terms:
  • Actins/genetics
  • Actins/metabolism
  • Animals
  • Animals, Genetically Modified
  • Bacterial Proteins/genetics
  • Bacterial Proteins/metabolism
  • Disease Models, Animal
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Genes, Reporter
  • Luminescent Proteins/genetics
  • Luminescent Proteins/metabolism
  • Membrane Proteins/antagonists & inhibitors
  • Membrane Proteins/genetics*
  • Membrane Proteins/metabolism
  • Mitochondria/metabolism*
  • Mitochondria/pathology
  • Mitochondrial Dynamics/genetics*
  • Morpholinos/genetics
  • Morpholinos/metabolism
  • Muscle Development/genetics
  • Muscle Proteins/antagonists & inhibitors
  • Muscle Proteins/genetics*
  • Muscle Proteins/metabolism
  • Muscle, Skeletal/metabolism*
  • Muscle, Skeletal/pathology
  • Muscular Dystrophies/genetics*
  • Muscular Dystrophies/metabolism
  • Muscular Dystrophies/pathology
  • NADP/metabolism
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Signal Transduction
  • Superoxide Dismutase/genetics
  • Superoxide Dismutase/metabolism
  • Time-Lapse Imaging
  • Zebrafish
  • Zebrafish Proteins/antagonists & inhibitors
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
PubMed: 26186000 Full text @ PLoS One
FIGURES
ABSTRACT
Mitochondrial flash (mitoflash) is a highly-conserved, universal, and physiological mitochondrial activity in isolated mitochondria, intact cells, and live organisms. Here we investigated developmental and disease-related remodeling of mitoflash activity in zebrafish skeletal muscles. In transgenic zebrafish expressing the mitoflash reporter cpYFP, in vivo imaging revealed that mitoflash frequency and unitary properties underwent multiphasic and muscle type-specific changes, accompanying mitochondrial morphogenesis from 2 to 14 dpf. In particular, short (S)-type mitoflashes predominated in early muscle formation, then S-, transitory (T)- and regular (R)-type mitoflashes coexisted during muscle maturation, followed by a switch to R-type mitoflashes in mature skeletal muscles. In early development of muscular dystrophy, we found accelerated S- to R-type mitoflash transition and reduced mitochondrial NAD(P)H amidst a remarkable cell-to-cell heterogeneity. This study not only unravels a profound functional and morphological remodeling of mitochondria in developing and diseased skeletal muscles, but also underscores mitoflashes as a useful reporter of mitochondrial function in milieu of live animals under physiological and pathophysiological conditions.
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