FIGURE
Fig. S6
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- ZDB-FIG-150831-6
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- Iyengar et al., 2015 - Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells
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Fig. S6
Treatment of differentiated melanocytes with the Wnt inhibitor IWR-1 (A) Flank of an adult zebrafish expressing Tg(miniCoopR-TOPFlash:mCherry) before (top row) and after (bottom row) treatment with IWR-1. Treatment with IWR-1 for 24 hours reduced mCherry signal in differentiated melanocytes. mC, miniCoopR; BF, brightfield. Scale bar = 100 µM. n = 3 fish; representative images are shown. (B) Representative images of adult wild-type zebrafish treated with DMSO alone or IWR-1 in DMSO without prior neocuproine-induced melanocyte ablation. Scale bar = 5mm. (C) Quantification of melanocytes (see Experimental Procedures) expressed as a ratio of melanocytes present at the specified time point to the number of melanocytes present initially. Data are shown as mean ± SEM. P values calculated by Student’s t-test, Day 7 P = 0.43, Day 10 P = 0.95, Day 12 P = 0.82, Day 15 P = 0.59. n = 2 fish. In (A) and (B) fish were treated with epinephrine before imaging. Related to Figure 7. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 33(6), Iyengar, S., Kasheta, M., Ceol, C.J., Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells, 631-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell