FIGURE

Fig. S5

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ZDB-FIG-150831-5
Publication
Iyengar et al., 2015 - Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells
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Fig. S5

TOPFlash reporter signal is lost when differentiated melanocytes are ablated

Flank of an adult Tg(mitfa:nlsEGFP); Tg(miniCoopR-TOPFlash:mCherry) zebrafish before (top row) and after (bottom row) neocuproine treatment to ablate differentiated melanocytes. Differentiated melanocytes (arrowheads) expressed nlsEGFP and mCherry, but both signals were lost in these cells within one day of neocuproine treatment. Fish were treated with epinephrine before imaging. mC, miniCoopR; mCh, mCherry; BF, brightfield. Scale bar = 100µM. n = 3 fish; representative images are shown. Related to Figure 6.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 33(6), Iyengar, S., Kasheta, M., Ceol, C.J., Poised Regeneration of Zebrafish Melanocytes Involves Direct Differentiation and Concurrent Replenishment of Tissue-Resident Progenitor Cells, 631-43, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell