ZFIN ID: ZDB-FIG-150713-6
Wong et al., 2015 - Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression. Developmental Biology   402(2):216-28 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 6

The Tg(hsp70l:dnfgfr1-EGFP) transgenic line provides a temporal model for loss of vsx2. Immunohistochemistry was performed on retinal sections of Dnfgfr1- no heat shock, Dnfgfr1-heat shock, Dnfgfr1+MO1a-no heat shock, and Dnfgfr1+MO1a-heat shock at 48 hpf. The markers used are Vsx2 (A–D) and Zn5 (E–H). Vsx2 expression was not completely rescued when both dmbx1 and FGF signaling was knocked down but differentiation of Zn5 positive ganglion cells was partially rescued.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
vsx2 pd1Tg control Long-pec retina central region IHC
pd1Tg heat shock Long-pec retina central region IHC
pd1Tg + MO1-dmbx1a control Long-pec retina IHC
Long-pec retina central region IHC
pd1Tg + MO1-dmbx1a heat shock Long-pec retina central region IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
zn-5 IHC pd1Tg control Long-pec retinal ganglion cell
IHC pd1Tg heat shock Long-pec retinal ganglion cell
IHC pd1Tg + MO1-dmbx1a control Long-pec Not Detected retina
IHC pd1Tg + MO1-dmbx1a heat shock Long-pec retinal ganglion cell
Phenotype Details
Fish Conditions Stage Phenotype
pd1Tg heat shock Long-pec eye decreased size, abnormal
Long-pec retina central region vsx2 expression decreased amount, abnormal
pd1Tg + MO1-dmbx1a control Long-pec retina zn-5 labeling absent, abnormal
Long-pec retina vsx2 expression increased amount, abnormal
Long-pec retina central region vsx2 expression increased amount, abnormal
Long-pec retinal ganglion cell absent, ameliorated
Long-pec retinal ganglion cell decreased amount, abnormal
pd1Tg + MO1-dmbx1a heat shock Long-pec retina central region vsx2 expression amount, ameliorated
pd1Tg + MO1-dmbx1a heat shock Long-pec retinal ganglion cell amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.