header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-150713-2
Wong et al., 2015 - Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression. Developmental Biology   402(2):216-28 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2

vsx2 gene expression in MO1a+MO1b embryos. In situ hybridization of vsx2 gene expression at 48 hpf (A and B). RT-qPCR was used to quantify RNA from three pools of 50 embryos collected from uninjected and morpholino injected embryos (C). There was a significant increase in vsx2 expression using Student′s t-test (p<0.05). Standard deviation was calculated from three replicate experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
vsx2 AB standard conditions Long-pec ciliary marginal zone ISH
AB + MO1-dmbx1a + MO1-dmbx1b standard conditions Long-pec ciliary marginal zone ISH
Long-pec head RTPCR
Long-pec retina central region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-dmbx1a + MO1-dmbx1b standard conditions Long-pec head vsx2 expression increased amount, abnormal
Long-pec retina central region vsx2 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 402(2), Wong, L., Power, N., Miles, A., Tropepe, V., Mutual antagonism of the paired-type homeobox genes, vsx2 and dmbx1, regulates retinal progenitor cell cycle exit upstream of ccnd1 expression, 216-28, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.