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ZFIN ID: ZDB-FIG-150625-14
Guiu et al., 2014 - Identification of Cdca7 as a novel Notch transcriptional target involved in hematopoietic stem cell emergence. The Journal of experimental medicine   211(12):2411-23 Full text @ J. Exp. Med.
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 5 HSC formation is impaired in cdca7 morphants. (A) cdca7 ISH of representative embryos treated at 6 hpf for 24 h with DMSO or 100 μM DAPT. (B) Representative embryos (top) and detailed images of AGM region (bottom) from embryos treated with control or cdca7 MOs. Hematopoietic cells were detected by cmyb ISH at 30 hpf or by CD41-GFP at 50 hpf (C). The number of CD41-GFP–positive cells is significantly reduced upon cdca7 MO injection (P = 0.01). (D) Representative images from CD41-GFP and Flt1-RFP embryos treated with control or cdca7 MOs. Number of double-positive cells is indicated. A detail of CD41-GFP+/Flt1-RFP+ HSCs is depicted in the inset. (E and F) The dorsal aorta integrity is revealed by gridlock (hey2) and EphrinB2a expression in both WT and cdca7 MO-injected embryos. The numbers in the panels indicate the number of embryos of the total pool that displayed phenotype or the number of positive cells. (G) The percentages of cmyb expression are indicated in the graph. Bars: (A, B, E, and F) 200 μm; (C and D) 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdca7a WT control Prim-15 ventral wall of dorsal aorta ISH
WT chemical treatment: DAPT Prim-15 Not Detected ventral wall of dorsal aorta ISH
efnb2a WT standard conditions Prim-15 dorsal aorta ISH
WT + MO2-cdca7a standard conditions Prim-15 dorsal aorta ISH
EGFP hu5333Tg; la2Tg standard conditions Long-pec hematopoietic stem cell IFL
Long-pec myeloid lineage restricted progenitor cell IFL
hu5333Tg; la2Tg + MO2-cdca7a standard conditions Long-pec hematopoietic stem cell IFL
Long-pec myeloid lineage restricted progenitor cell IFL
la2Tg standard conditions Long-pec hematopoietic stem cell IFL
Long-pec myeloid lineage restricted progenitor cell IFL
la2Tg + MO2-cdca7a standard conditions Long-pec hematopoietic stem cell IFL
Long-pec myeloid lineage restricted progenitor cell IFL
hey2 WT standard conditions Prim-15 dorsal aorta ISH
WT + MO2-cdca7a standard conditions Prim-15 dorsal aorta ISH
myb WT standard conditions Prim-15 hematopoietic multipotent progenitor cell ISH
Prim-15 hematopoietic stem cell ISH
WT + MO2-cdca7a standard conditions Prim-15 Not Detected hematopoietic multipotent progenitor cell ISH
Prim-15 Not Detected hematopoietic stem cell ISH
RFP hu5333Tg; la2Tg standard conditions Long-pec dorsal aorta IFL
Long-pec hematopoietic stem cell IFL
Long-pec intersegmental artery IFL
hu5333Tg; la2Tg + MO2-cdca7a standard conditions Long-pec dorsal aorta IFL
Long-pec hematopoietic stem cell IFL
Long-pec intersegmental artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: DAPT Prim-15 Notch signaling pathway decreased occurrence, abnormal
Prim-15 ventral wall of dorsal aorta physical object quality, abnormal
WT + MO2-cdca7a standard conditions Prim-15 artery development process quality, normal
Prim-15 hematopoietic stem cell differentiation decreased occurrence, abnormal
hu5333Tg; la2Tg + MO2-cdca7a standard conditions Long-pec hematopoietic stem cell differentiation decreased occurrence, abnormal
Long-pec whole organism has fewer parts of type hematopoietic stem cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal The Journal of experimental medicine for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ J. Exp. Med.