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ZFIN ID: ZDB-FIG-150319-8
Takeuchi et al., 2015 - Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Developmental Biology   397(1):1-17 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult

Fig. 4 Purkinje-specific GFF and membrane tagged Venus/mCherry lines. (A–H) aldoca:gap43-Venus larvae (A–D, dorsal views with anterior to the left) and adult sagittal sections (E–H, with anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. Venus signals colocalized with Pvalb7 signals in all the Purkinje cells of 5-dpf larvae (D) and the adult cerebellum (H). Cerebellovestibular tracts are indicated by arrowheads (A, B). (I–L) aldoca:gap43-mCherry larvae (dorsal views with anterior to the left) were co-stained with anti-DsRed (mCherry, magenta) and anti-Pvalb7 (green) antibodies. (M–S) aldoca:GFF; UAS:GFP larvae (M–P, dorsal views with anterior to the left) and adult sagittal sections (Q–V, anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. (T–V) High magnification views of the box in S. Only a portion of the Pvalb7+ Purkinje cells stained positive for GFP (L, P). Scale bars: 100 µm in A; 50 µm in D (applied to B–D); 200 µm in E; 50 µm in H (applied to F–H); 100 µm in I; 50 µm in L (applied to J–L); 100 µm in M; 50 µm in P (applied to N–P); 200 µm in S (applied to Q–S); 100 µm in V (applied to T–V).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkuasgfp1aTg; nub6Tg control Day 5 Purkinje cell IHC
Adult Purkinje cell IHC
mCherry nub5Tg control Day 5 Purkinje cell IHC
Venus rk22Tg control Day 5 cerebellovestibular tract IHC
Day 5 Purkinje cell IHC
Adult Purkinje cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-pvalb7 IHC nkuasgfp1aTg; nub6Tg control Day 5 Purkinje cell
IHC Adult Purkinje cell
IHC nub5Tg control Day 5 Purkinje cell
IHC rk22Tg control Day 5 Purkinje cell
IHC Adult Purkinje cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.