|ZFIN ID: ZDB-FIG-150319-8|
|Stage Range:||Day 5 to Adult|
Fig. 4 Purkinje-specific GFF and membrane tagged Venus/mCherry lines. (A–H) aldoca:gap43-Venus larvae (A–D, dorsal views with anterior to the left) and adult sagittal sections (E–H, with anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. Venus signals colocalized with Pvalb7 signals in all the Purkinje cells of 5-dpf larvae (D) and the adult cerebellum (H). Cerebellovestibular tracts are indicated by arrowheads (A, B). (I–L) aldoca:gap43-mCherry larvae (dorsal views with anterior to the left) were co-stained with anti-DsRed (mCherry, magenta) and anti-Pvalb7 (green) antibodies. (M–S) aldoca:GFF; UAS:GFP larvae (M–P, dorsal views with anterior to the left) and adult sagittal sections (Q–V, anterior to the left) were co-stained with anti-GFP (Venus, green) and anti-Pvalb7 (magenta) antibodies. (T–V) High magnification views of the box in S. Only a portion of the Pvalb7+ Purkinje cells stained positive for GFP (L, P). Scale bars: 100 µm in A; 50 µm in D (applied to B–D); 200 µm in E; 50 µm in H (applied to F–H); 100 µm in I; 50 µm in L (applied to J–L); 100 µm in M; 50 µm in P (applied to N–P); 200 µm in S (applied to Q–S); 100 µm in V (applied to T–V).
|EGFP||nkuasgfp1aTg; nub6Tg||control||Day 5||Purkinje cell||IHC|
|mCherry||nub5Tg||control||Day 5||Purkinje cell||IHC|
|Venus||rk22Tg||control||Day 5||cerebellovestibular tract||IHC|
|Day 5||Purkinje cell||IHC|
|Ab1-pvalb7||IHC||nkuasgfp1aTg; nub6Tg||control||Day 5||Purkinje cell|
|IHC||nub5Tg||control||Day 5||Purkinje cell|
|IHC||rk22Tg||control||Day 5||Purkinje cell|
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.