ZFIN ID: ZDB-FIG-150319-6
Takeuchi et al., 2015 - Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry. Developmental Biology   397(1):1-17 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 5 to Adult

Fig. 2 Granule-specific GFF and GFP transgenic lines. (A, B) gSA2AzGFF152B; UAS:GFP. (C, D) gSAIzGFFM765B; UAS:GFP. (E, F) gSAG6A. (G, H) gSAIGFF23C; UAS:GFP. (I, J) SAGFF(LF)128A; UAS:GFP. (K, L) hspGFF57A; UAS:GFP. (M, N) SAGFF(LF)157B; UAS:GFP. (O, P) hspGFFDMC90A; UAS:GFP. To visualize GFF expression, the granule-specific GFF lines (except gSAG6A in E, F) were crossed with the UAS:GFP line. The 5-dpf larvae (A, C, E, G, I, K, M, O, dorsal projection views with anterior to the left) and sagittal sections of adult brains (B, D, F, H, J, L, N, P, optical sections with anterior to the left) were stained with anti-GFP antibody. Note that in the gSA2AzGFF152B; UAS:GFP line GFP was specifically expressed in the granule cells of the CCe, while in the gSAIzGFFM765B; UAS:GFP line GFP was expressed in some granule cells in the CCe and most granule cells in the LCa in adult. The gSAG6A and gSAIGFF23C; UAS:GFP lines expressed GFP in granule cells primarily in the LCa. In the hspGFFDMC90A; UAS:GFP line GFP was expressed in some granule cells in the GCL and LCa, and in some immature granule cells in the ML. Scale bars: 100 µm in O (applied to A, C, E, G, I, K, M, O); 200 µm in B, D, F, H, J, L, N, P.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkSAGFFLF128AEt; nkuasgfp1aTg control Day 5 cerebellar granule cell IHC
Adult cerebellar granule cell IHC
nkSAGFFLF157BEt; nkuasgfp1aTg control Day 5 cerebellar granule cell IHC
Adult cerebellar granule cell IHC
nkgSA2AzGFF152BGt; nkuasgfp1aTg control Day 5 cerebellar granule cell IHC
Day 5 corpus cerebelli cerebellar granule cell IHC
Adult cerebellar crest cerebellar granule cell IHC
Adult cerebellar granule cell IHC
Adult granular layer corpus cerebelli cerebellar granule cell IHC
Adult granular layer valvula cerebelli cerebellar granule cell IHC
Adult valvula cerebelli cerebellar granule cell IHC
nkgSAG6AGt control Day 5 cerebellar granule cell IHC
Day 5 medial caudal lobe cerebellar granule cell IHC
Adult cerebellar granule cell IHC
Adult medial caudal lobe cerebellar granule cell IHC
nkgSAIGFF23CGt; nkuasgfp1aTg control Day 5 cerebellar granule cell IHC
Day 5 medial caudal lobe cerebellar granule cell IHC
Adult cerebellar granule cell IHC
Adult medial caudal lobe cerebellar granule cell IHC
nkgsaizGFFM765BGt/nkgsaizGFFM765BGt; nkuasgfp1aTg control Day 5 cerebellar granule cell IHC
Day 5 corpus cerebelli cerebellar granule cell IHC
Day 5 medial caudal lobe cerebellar granule cell IHC
Adult cerebellar crest cerebellar granule cell IHC
Adult cerebellar granule cell IHC
Adult medial caudal lobe cerebellar granule cell IHC
nkhspGFF57AEt; nkuasgfp1aTg heat shock Day 5 cerebellar granule cell IHC
Adult cerebellar granule cell IHC
nkhspGFFDMC90AEt; nkuasgfp1aTg heat shock Day 5 cerebellar crest cerebellar granule cell IHC
Day 5 cerebellar granule cell IHC
Day 5 corpus cerebelli cerebellar granule cell IHC
Day 5 medial caudal lobe cerebellar granule cell IHC
Adult cerebellar crest cerebellar granule cell IHC
Adult cerebellar granule cell IHC
Adult corpus cerebelli cerebellar granule cell IHC
Adult medial caudal lobe cerebellar granule cell IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 397(1), Takeuchi, M., Matsuda, K., Yamaguchi, S., Asakawa, K., Miyasaka, N., Lal, P., Yoshihara, Y., Koga, A., Kawakami, K., Shimizu, T., Hibi, M., Establishment of Gal4 transgenic zebrafish lines for analysis of development of cerebellar neural circuitry, 1-17, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.