ZFIN ID: ZDB-FIG-150108-4
Huang et al., 2013 - Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis. Developmental Biology   384(2):290-300 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 5-9 somites to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 5

PGP midline convergence phenotypes in ntl-/- embryos. ((A)-(E)) Whole-mount in situ hybrydization of wt1a and myoD was stained blue and orange, respectively in 12 (A), 24 (B) and 48 hpf (C) ntl-/- embryos. The scale bar indicates 100 ┬Ám; ((A)-(C)) are at the same magnification. All images are dorsal view anterior to the top. (D) Quantification of convergence morphogenetic movements in ntl-/- embryos and comparison with WT. The inter distance of PGP was measured at 11 (n=20, P=0.68), 12 (n=17, P=0.27), 24 (n=23,), 36 (n=29), 48 (n=20) hpf. The inter distance of PGP is significant difference between ntl-/- and WT at 24, 36 and 48 hpf (***P<0.0005). Error bars represent SD.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 tbxtab195/b195 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
Prim-5 adaxial cell ISH
Prim-5 somite ISH
Long-pec somite ISH
tbxtatc41/tc41 standard conditions 5-9 somites adaxial cell ISH
5-9 somites somite ISH
Prim-5 adaxial cell ISH
Prim-5 somite ISH
Long-pec somite ISH
wt1a tbxtab195/b195 standard conditions 5-9 somites pronephric mesoderm ISH
Prim-5 pronephric glomerulus ISH
Long-pec pronephric glomerulus ISH
tbxtatc41/tc41 standard conditions 5-9 somites pronephric mesoderm ISH
Prim-5 pronephric glomerulus ISH
Long-pec pronephric glomerulus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tbxtab195/b195 standard conditions Prim-5 convergent extension involved in nephron morphogenesis process quality, abnormal
Prim-5 pronephric glomerulus morphogenesis process quality, abnormal
Long-pec convergent extension involved in nephron morphogenesis process quality, abnormal
Long-pec pronephric glomerulus morphogenesis process quality, abnormal
tbxtatc41/tc41 standard conditions Prim-5 convergent extension involved in nephron morphogenesis process quality, abnormal
Prim-5 pronephric glomerulus morphogenesis process quality, abnormal
Long-pec convergent extension involved in nephron morphogenesis process quality, abnormal
Long-pec pronephric glomerulus morphogenesis process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 384(2), Huang, C.J., Wilson, V., Pennings, S., MacRae, C.A., and Mullins, J., Sequential effects of spadetail, one-eyed pinhead and no tail on midline convergence of nephric primordia during zebrafish embryogenesis, 290-300, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.