ZFIN ID: ZDB-FIG-140522-47
Markmiller et al., 2014 - Minor class splicing shapes the zebrafish transcriptome during development. Proceedings of the National Academy of Sciences of the United States of America   111(8):3062-3067 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 The clbn phenotype is characterized by abnormalities in the digestive organs (A and B) Brightfield images of left lateral views and (C) histology of WT and clbns846 larvae at 120 hpf show a thick, folded intestinal epithelium (black arrows in C) and inflated swim bladder in WT, whereas clbn displays a thin, unfolded intestinal epithelium, delayed yolk resorption, and smaller eyes. (D) Two-photon microscopy of WT and clbns846 larvae on the Tg(gutGFP)s854 background and (E) epifluorescence microscopy on the Tg(fabp10:RFP,ela3l:EGFP)gz12 background reveal smaller digestive organs in clbns846 compared with WT. The two images in A were taken at the same magnification, as were the two images in each of panels B, D, and E. The clbn phenotype is 100% penetrant and all larvae die between 7 and 10 dpf; however, the severity of the morphological abnormalities may vary between clutches. For example, the digestive organ phenotype is visibly conspicuous in some clutches at around 108 hpf and not in others until 120 hpf. a, anus; e, eye; es, esophagus; ib, intestinal bulb; Li, liver; p, pancreas; sb, swim bladder; y, yolk. (Scale bar in C, 100 ┬Ám.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg standard conditions Day 5 liver IFL
rnpc3s846/s846; gz15Tg standard conditions Day 5 liver IFL
EGFP gz15Tg standard conditions Day 5 pancreas IFL
rnpc3s846/s846; gz15Tg standard conditions Day 5 pancreas IFL
GFP rnpc3s846/s846; s854Tg standard conditions Day 5 liver IFL
s854Tg standard conditions Day 5 liver IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rnpc3s846/s846 standard conditions Day 5 eye decreased size, abnormal
Day 5 intestinal epithelium decreased thickness, abnormal
Day 5 intestinal epithelium surface feature shape, abnormal
Day 5 swim bladder malformed, abnormal
Day 5 yolk physical object quality, abnormal
rnpc3s846/s846; gz15Tg standard conditions Day 5 liver decreased size, abnormal
Day 5 pancreas decreased size, abnormal
rnpc3s846/s846; s854Tg standard conditions Day 5 liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA