buc transgenes lacking introns disrupt RNA localization. (A) RT-PCR on ovary lysate cDNA. Transgenic and endogenous transcript expression in ovaries of F1 transgenic females expressing intron-lacking transgenes in bucp106/+ heterozygotes. (B,C) buc RNA in ovaries of cbuc80+;bucp106/+ F1 transgenic females (B) resembles WT or (C) is not asymmetrically localized. (D) Quantification of buc RNA expression patterns in stage I oocytes. (E,F) vasa expression in (E) F1 transgenic- and (F) cbuc80 F1 transgenic+ ovaries. (B,E) Blue arrows indicate the Bb. (G) Quantification of vasa expression patterns. (H-J) Relative expression of (H) cbuc80, (I) endogenous buc and (J) vasa in sorted oocytes of F1 or F2 females. (D,G) het, bucp106/+; mut, bucp106/p106; wt, buc+/+.