ZFIN ID: ZDB-FIG-140506-20
Heim et al., 2014 - Oocyte polarity requires a Bucky ball-dependent feedback amplification loop. Development (Cambridge, England)   141(4):842-854 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult

Fig. 5

buc transgenes lacking introns disrupt RNA localization. (A) RT-PCR on ovary lysate cDNA. Transgenic and endogenous transcript expression in ovaries of F1 transgenic females expressing intron-lacking transgenes in bucp106/+ heterozygotes. (B,C) buc RNA in ovaries of cbuc80+;bucp106/+ F1 transgenic females (B) resembles WT or (C) is not asymmetrically localized. (D) Quantification of buc RNA expression patterns in stage I oocytes. (E,F) vasa expression in (E) F1 transgenic- and (F) cbuc80 F1 transgenic+ ovaries. (B,E) Blue arrows indicate the Bb. (G) Quantification of vasa expression patterns. (H-J) Relative expression of (H) cbuc80, (I) endogenous buc and (J) vasa in sorted oocytes of F1 or F2 females. (D,G) het, bucp106/+; mut, bucp106/p106; wt, buc+/+.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
buc bucp106re/+; ae7Tg standard conditions Adult ovary ISH
ddx4 bucp106re/+ standard conditions Adult ovary ISH
bucp106re/+; ae7Tg standard conditions Adult ovary ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development