ZFIN ID: ZDB-FIG-140506-20
Heim et al., 2014 - Oocyte polarity requires a Bucky ball-dependent feedback amplification loop. Development (Cambridge, England)   141(4):842-854 Full text @ Development
Anatomical Term:
Stage: Adult

Fig. 5

buc transgenes lacking introns disrupt RNA localization. (A) RT-PCR on ovary lysate cDNA. Transgenic and endogenous transcript expression in ovaries of F1 transgenic females expressing intron-lacking transgenes in bucp106/+ heterozygotes. (B,C) buc RNA in ovaries of cbuc80+;bucp106/+ F1 transgenic females (B) resembles WT or (C) is not asymmetrically localized. (D) Quantification of buc RNA expression patterns in stage I oocytes. (E,F) vasa expression in (E) F1 transgenic- and (F) cbuc80 F1 transgenic+ ovaries. (B,E) Blue arrows indicate the Bb. (G) Quantification of vasa expression patterns. (H-J) Relative expression of (H) cbuc80, (I) endogenous buc and (J) vasa in sorted oocytes of F1 or F2 females. (D,G) het, bucp106/+; mut, bucp106/p106; wt, buc+/+.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
buc bucp106re/+; ae7Tg standard conditions Adult ovary ISH
ddx4 bucp106re/+ standard conditions Adult ovary ISH
bucp106re/+; ae7Tg standard conditions Adult ovary ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development