ZFIN ID: ZDB-FIG-140506-16
Heim et al., 2014 - Oocyte polarity requires a Bucky ball-dependent feedback amplification loop. Development (Cambridge, England)   141(4):842-854 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 1

Buc protein localizes to Balbiani bodies (Bbs) and is asymmetric before Bb formation. (A,A2,C) Buc protein localization in Bbs (red arrows in A,C) of stage Iaz (zygotene), Iap (pachytene) and Ib (larger than Ia, arrested in diplotene) WT oocytes in whole-mount ovaries stained with anti-Buc antibodies. Buc protein is not detected in bucp106/p106 mutants (B,B2). (A2,B2) Tracings of the oocytes (blue lines) and their nuclei (yellow dashed lines) from A and B. (D) DAPI-labeled nuclei of oocytes (yellow dashed circles) and follicle cells (blue arrows). (E) Merge of C and D. (F) Corresponding phase image to E. (G) Perinuclear localization of Buc (pink arrows) in WT stage Iap oocytes before Bb formation. (G2) Tracing of oocytes (blue lines) and nuclei (yellow lines). n/nuc, nucleus.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-buc IHC WT standard conditions Adult oocyte mitochondrial cloud
IHC Adult oocyte nuclear envelope lumen
IHC Adult oocyte stage I mitochondrial cloud
IHC bucp106re/p106re standard conditions Adult Not Detected oocyte
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development