ZFIN ID: ZDB-FIG-140506-19
Heim et al., 2014 - Oocyte polarity requires a Bucky ball-dependent feedback amplification loop. Development (Cambridge, England)   141(4):842-854 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4

Defective Bb formation and excess polarized somatic fates in transgenics lacking introns. (A-D) Oocytes from cbuc80 transgenic founders. (A-F) Hematoxylin and Eosin (H&E)-stained F0 ovary sections reveal a normal composition of oocytes, including (A) stage III oocytes with single micropylar cells (arrow and a1), (B) stage III oocytes with multiple micropylar cells (arrows and b1 and b2), (C) stage I oocytes with Bbs and (D) stage I oocytes lacking Bbs. (E,F) Ectopic Bbs of cbuc+ F1 females. (G-J) DiOC6 staining of sectioned ovaries. cbuc80 F1 ovaries reveal primary oocytes with (G) and without (H) Bbs. (I,J) Ectopic Bbs of cbuc+ F1 females. (K) BF view of oocyte in J. Arrows indicate Bbs. (L) Quantification of Bbs from different individual F1 transgenic females labeled with DiOC6. Cg, cortical granules; n, nucleus.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ae6Tg standard conditions Adult oocyte mitochondrial cloud increased amount, abnormal
ae7Tg standard conditions Adult oocyte stage I mitochondrial cloud absent, abnormal
Adult oocyte stage III micropylar cell increased amount, abnormal
bucp106re/+ standard conditions Adult oocyte mitochondrial cloud absent, abnormal
bucp106re/+; ae7Tg standard conditions Adult oocyte mitochondrial cloud absent, abnormal
bucp106re/p106re standard conditions Adult oocyte mitochondrial cloud absent, abnormal
bucp106re/p106re; ae7Tg standard conditions Adult oocyte mitochondrial cloud absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development