ZFIN ID: ZDB-FIG-140319-66
Scholpp et al., 2001 - Morpholino-induced knockdown of zebrafish engrailed genes eng2 and eng3 reveals redundant and unique functions in midbrain–hindbrain boundary development. Genesis (New York, N.Y. : 2000)   30(3):129-133 Full text @ Genesis
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Bud to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 1 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en2b WT control 5-9 somites midbrain hindbrain boundary neural keel ISH
20-25 somites midbrain hindbrain boundary ISH
20-25 somites optic stalk ISH
20-25 somites otic vesicle ISH
WT + MO1-en2a standard conditions 5-9 somites midbrain hindbrain boundary neural keel ISH
20-25 somites midbrain hindbrain boundary ISH
20-25 somites optic stalk ISH
20-25 somites otic vesicle ISH
WT + MO1-en2a + MO1-en2b standard conditions 20-25 somites Not Detected midbrain hindbrain boundary ISH
20-25 somites optic stalk ISH
20-25 somites otic vesicle ISH
fgf8a WT + MO1-en2a + MO1-en2b standard conditions Bud midbrain hindbrain boundary neural keel ISH
pax2a WT + MO1-en2a + MO1-en2b standard conditions Bud midbrain hindbrain boundary neural keel ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-eng IHC WT control 10-13 somites midbrain hindbrain boundary neural keel
IHC WT + MO1-en2a + MO1-en2b standard conditions 10-13 somites Not Detected midbrain hindbrain boundary neural keel
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-en2a standard conditions Prim-5 optic tectum decreased size, abnormal
WT + MO1-en2a + MO1-en2b standard conditions Prim-5 brain lacks all parts of type midbrain hindbrain boundary, abnormal
Prim-5 brain lacks all parts of type optic tectum, abnormal
Prim-5 midbrain-hindbrain boundary development disrupted, abnormal
Full text @ Genesis