FIGURE

Fig. 2

ID

ZDB-FIG-140117-4

Publication

Thi Thu et al., 2013 - tbx2a Is Required for Specification of Endodermal Pouches during Development of the Pharyngeal Arches

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Fig. 2

Morpholino activity.

(A) 48 hpf live MO1/MO2/MO3-injected embryos (doses of 0.5 pmole/0.1 pmole/0.1 pmole, respectively) exhibit hydrocephalus, heart edema (arrow), body curvature, and reductions to the ears and eyes (arrow). (B) Amplified mature transcripts of MO2 morphants on a 0.8% agarose gel. Only one mature transcript is present in the WT, with reduction of the transcript detected in morphants injected with MO2. An additional small transcript was detected caused by the binding of MO2 to the acceptor site of intron 1 and excision of a fragment containing intron 1, exon 2 and intron 2. (C) Mature transcript results from joining of exon 1 and exon 3 and a frame shift-induced stop codon (TAA-black box) at the beginning of exon 3. cx43a expression is normally absent in the ventral diencephalon of control embryos (D), but is ectopically activated in the tbx2a morphants, (E) in the domain of tbx2a expression, and (F) in the ventral diencephalon.

Expression Data

Genes:	gja1b tbx2a
Fish:	WT WT + MO4-tbx2a
Knockdown Reagent:	MO4-tbx2a
Anatomical Term:	hypothalamus
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO3-tbx2a WT + MO4-tbx2a WT + MO5-tbx2a
Knockdown Reagents:	MO3-tbx2a MO4-tbx2a MO5-tbx2a
Observed In:	ball eye heart inner ear pharyngeal arch proctodeum swim bladder inflation whole organism axis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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