Par3 is required for NC migration in zebrafish. (A) Dorsal view of 5-somite stage zebrafish embryos injected unilaterally with ControlMO or Par3MO and processed for in situ hybridisation against foxd3; no effect on NC induction was observed (n=72). (B) Schematic representation of zebrafish embryo indicating the regions shown in C-H. (C,D) Lateral views showing foxd3 expression in the trunk of ControlMO-injected (C) or Par3MO-injected (D) 20-somite embryos. Arrows indicate distinct trunk NC streams. (E,F) Lateral views showing heads of 24-hour sox10:egfp embryos injected with ControlMO (E) or Par3MO (F). OV, otic vesicle. Arrows indicate distinct cranial NC streams. Lines indicate that no distinct streams are observed. (G,H) Single frames from time-lapse movies showing one cranial NC stream and corresponding Delaunay triangulation at 0 hours (15 somites) and 6 hours from ControlMO-injected (G) and Par3MO-injected (H) embryos. (I) Distance between cranial NC cells analysed by nearest neighbour computation. n=15 embryos for each condition, with more than 30 cells analysed per explant; **P<0.01, ***P<0.001. Error bars indicate s.d.