ZFIN ID: ZDB-FIG-140117-24
Moore et al., 2013 - Par3 controls neural crest migration by promoting microtubule catastrophe during contact inhibition of locomotion. Development (Cambridge, England)   140(23):4763-4775 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 5-9 somites to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 14-19 somites to Prim-15

Fig. 2

Par3 is required for NC migration in zebrafish. (A) Dorsal view of 5-somite stage zebrafish embryos injected unilaterally with ControlMO or Par3MO and processed for in situ hybridisation against foxd3; no effect on NC induction was observed (n=72). (B) Schematic representation of zebrafish embryo indicating the regions shown in C-H. (C,D) Lateral views showing foxd3 expression in the trunk of ControlMO-injected (C) or Par3MO-injected (D) 20-somite embryos. Arrows indicate distinct trunk NC streams. (E,F) Lateral views showing heads of 24-hour sox10:egfp embryos injected with ControlMO (E) or Par3MO (F). OV, otic vesicle. Arrows indicate distinct cranial NC streams. Lines indicate that no distinct streams are observed. (G,H) Single frames from time-lapse movies showing one cranial NC stream and corresponding Delaunay triangulation at 0 hours (15 somites) and 6 hours from ControlMO-injected (G) and Par3MO-injected (H) embryos. (I) Distance between cranial NC cells analysed by nearest neighbour computation. n=15 embryos for each condition, with more than 30 cells analysed per explant; **P<0.01, ***P<0.001. Error bars indicate s.d.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg control 14-19 somites cranial neural crest cell IFL
Prim-5 cranial neural crest cell IFL
Prim-15 neural crest cell IFL
ba2Tg + MO1-pard3ab standard conditions 14-19 somites cranial neural crest cell IFL
Prim-5 cranial neural crest cell IFL
Prim-15 neural crest cell IFL
foxd3 WT control 5-9 somites neural crest cell ISH
20-25 somites neural crest cell ISH
WT + MO1-pard3ab standard conditions 5-9 somites trunk neural crest cell ISH
20-25 somites trunk neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-pard3ab standard conditions 20-25 somites trunk neural crest cell migration decreased process quality, abnormal
ba2Tg + MO1-pard3ab standard conditions 14-19 somites - Prim-15 neural crest cell migration decreased process quality, abnormal
Prim-5 neural crest cell migration decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development