ZFIN ID: ZDB-FIG-140117-27
Moore et al., 2013 - Par3 controls neural crest migration by promoting microtubule catastrophe during contact inhibition of locomotion. Development (Cambridge, England)   140(23):4763-4775 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 20-25 somites to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Prim-5

Fig. 5

Par3 is required for CIL in vivo in zebrafish. Single frames from time-lapse movies illustrating a collision between two cranial NC cells (1 and 2) in a zebrafish embryo injected with ControlMO (A,B) or Par3MO (D,E). Images are overlapped with tracks of migratory paths (A,D) or the difference in protrusion between current and previous frames (B,E). Red area indicates new protrusions. (C,F) Velocity vectors of colliding NC cells in ControlMO-injected (C) or Par3MO-injected (F) embryos. Cell position was recorded at 5-minute intervals. Velocity change was clustered in ControlMO-injected cells (n=10; P<0.05), but no difference in the velocity change was observed in Par3MO-injected cells (n=9; P>0.1). Red arrow indicates the initial velocity vector.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg control 20-25 somites to Prim-5 cranial neural crest cell IFL
ba2Tg + MO1-pard3ab standard conditions 20-25 somites to Prim-5 cranial neural crest cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ba2Tg + MO1-pard3ab standard conditions 20-25 somites - Prim-5 neural crest cell migration process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development