ZFIN ID: ZDB-FIG-131218-40
Targoff et al., 2013 - Nkx genes are essential for maintenance of ventricular identity. Development (Cambridge, England)   140(20):4203-4213 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Long-pec

Fig. 1 Mutation of nkx2.5 disrupts cardiac morphogenesis. (A,B) Lateral views of live zebrafish embryos, anterior to the left, at 52 hpf. Other than cardiac defects and pericardial edema, the nkx2.5 mutant embryo (B) appears morphologically normal. (C,D) Lateral views of live embryos, anterior to the top, at 52 hpf. In contrast to the wild-type heart (C), the nkx2.5 mutant heart (D) is unlooped and has striking defects in both ventricular and atrial morphology. (E,F) MF20/S46 immunofluorescence distinguishes ventricular myocardium (red) from atrial myocardium (yellow). Frontal views, anterior to the top, at 52 hpf. In comparison to the wild-type heart (E), the nkx2.5 mutant heart (F) has a diminutive ventricle and an enlarged atrium. (G-L) In situ hybridization depicts expression of myl7 (G,H), vmhc (I,J) and amhc (K,L) in wild-type (G,I,K) and nkx2.5 mutant (H,J,L) embryos. Dorsal views, anterior to the top, at 26 hpf. (G,H) nkx2.5 mutant embryos exhibit subtle defects in heart tube extension, including a broad inflow region and a compact outflow region. In nkx2.5 mutants, the ventricular portion of the heart tube is abnormally short and wide (I,J) and the atrial portion of the heart tube has a splayed appearance (K,L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myh6 WT standard conditions Prim-5 atrial myocardium ISH
nkx2.5vu179/vu179 standard conditions Prim-5 atrial myocardium ISH
myh7 WT standard conditions Prim-5 ventricular myocardium ISH
nkx2.5vu179/vu179 standard conditions Prim-5 ventricular myocardium ISH
myl7 WT standard conditions Prim-5 myocardium ISH
nkx2.5vu179/vu179 standard conditions Prim-5 myocardium ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC nkx2.5vu179/vu179 standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
Ab-S46 IHC WT standard conditions Long-pec atrial myocardium
IHC nkx2.5vu179/vu179 standard conditions Long-pec atrial myocardium
Phenotype Details
Fish Conditions Stage Phenotype
nkx2.5vu179/vu179 standard conditions Prim-5 atrial myocardium distended, abnormal
Prim-5 atrial myocardium increased width, abnormal
Prim-5 ventricular myocardium decreased size, abnormal
Long-pec atrial myocardium bulbous, abnormal
Long-pec atrial myocardium dilated, abnormal
Long-pec heart morphology, abnormal
Long-pec pericardial cavity edematous, abnormal
Long-pec ventricular myocardium decreased size, abnormal
Long-pec ventricular myocardium decreased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development