ZFIN ID: ZDB-FIG-131218-43
Targoff et al., 2013 - Nkx genes are essential for maintenance of ventricular identity. Development (Cambridge, England)   140(20):4203-4213 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 4 nkx2.5-/-;nkx2.7-/- double mutant demonstrates the synergistic requirement for Nkx genes in maintenance of ventricular identity. (A,B) Lateral views of live embryos, anterior to the left, at 52 hpf. General embryonic morphology appears similar in wild-type (A) and nkx2.7 mutant (B) embryos. (C,D) Lateral views of live embryos, anterior to the top, at 52 hpf. The nkx2.7 mutant heart (D) exhibits subtle defects in ventricular and atrial morphology. (E-I) Frontal views, anterior to the top, of MF20/S46 immunofluorescence, which distinguishes ventricular myocardium (red) from atrial myocardium (yellow), at 52 hpf. In comparison to wild-type embryos (E), nkx2.7 mutants (F) exhibit subtle abnormalities in chamber size and shape whereas nkx2.5 mutants (G) demonstrate an impressive size discrepancy between the ventricular and atrial chambers. (H) In the nkx2.5 mutant, loss of a single allele of nkx2.7 leads to increased localization of Amhc (S46, yellow) within the ventricle. (I) Exacerbation of the ventricular deficiency is evident in the nkx2.5-/-;nkx2.7-/- mutants, and Amhc is present throughout the entire cardiac structure.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC nkx2.5vu179/vu179 standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC nkx2.5vu179/vu179; nkx2.7vu413/+ standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC nkx2.5vu179/vu179; nkx2.7vu413/vu413 standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
IHC nkx2.7vu413/vu413 standard conditions Long-pec atrial myocardium
IHC Long-pec ventricular myocardium
Ab-S46 IHC WT standard conditions Long-pec atrial myocardium
IHC nkx2.5vu179/vu179 standard conditions Long-pec atrial myocardium
IHC nkx2.5vu179/vu179; nkx2.7vu413/+ standard conditions Long-pec atrial myocardium
IHC nkx2.5vu179/vu179; nkx2.7vu413/vu413 standard conditions Long-pec atrial myocardium
IHC nkx2.7vu413/vu413 standard conditions Long-pec atrial myocardium
Phenotype Details
Fish Conditions Stage Phenotype
nkx2.5vu179/vu179 standard conditions Long-pec atrial myocardium increased size, abnormal
Long-pec ventricular myocardium decreased size, abnormal
nkx2.5vu179/vu179; nkx2.7vu413/+ standard conditions Long-pec atrial myocardium increased size, abnormal
Long-pec ventricular myocardium decreased size, abnormal
nkx2.5vu179/vu179; nkx2.7vu413/vu413 standard conditions Long-pec atrial myocardium increased size, abnormal
Long-pec ventricular myocardium hypoplastic, abnormal
nkx2.7vu413/vu413 standard conditions Long-pec heart morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development