header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-130907-5
Alunni et al., 2013 - Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium. Development (Cambridge, England)   140(16):3335-47 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Adult

Fig. 5

Notch3 inhibition accounts for the effect of Notch blockade on RG activation. (A-F) Triple immunohistochemistry for the RG marker glutamine synthetase (GS, green), MCM5 (magenta) and HuC/D (blue) on telencephalic cross-sections from adult notch3+/+ siblings and notch3fh332/+ heterozygotes under control conditions (top row) or upon LY treatment (middle and bottom rows). Scale bar: 20 μm. Confocal projection images from four optical planes, each 1 μm thick. (G,H) Total number of MCM5-positive progenitors per section (G) and proportion of RG cells in proliferation (H) in the different genotypes and treatment conditions, as well as in the standard AB wild-type line. P<0.0001 (n=3 brains for AB, notch3+/+ and notch3fh332/+, respectively). (I) Schematic of the notch3-MO knock-down experiment: a BrdU pulse is applied 2 days after electroporation (EP) of fluorescein-labeled notch3-MO or control-MO in pallial ventricular cells. Brains are analyzed immediately. (J,K) Analysis of the proliferation status (anti-BrdU) (blue) of electroporated (fluorescein-positive) (green) RG (anti-S100β) (magenta), assessed by immunocytochemistry. Arrows indicate fluorescein-labeled radial glia BrdU-positive cells. Scale bar: 20 μm. Confocal projection images from four optical planes, each 1 μm thick. (L) Proportion of BrdU-positive cells within the radial glia MO-targeted population. P<0.001 (n=3 brains for each condition).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mcm5 AB control Adult dorsal telencephalon radial glial cell IHC
AB chemical treatment: LY-411575 Adult dorsal telencephalon radial glial cell IHC
notch3fh332/+ control Adult dorsal telencephalon radial glial cell IHC
notch3fh332/+ chemical treatment: LY-411575 Adult dorsal telencephalon radial glial cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC AB control Adult dorsal telencephalon neuron
IHC AB chemical treatment: LY-411575 Adult dorsal telencephalon neuron
IHC notch3fh332/+ control Adult dorsal telencephalon neuron
IHC notch3fh332/+ chemical treatment: LY-411575 Adult dorsal telencephalon neuron
Ab1-glul IHC AB control Adult dorsal telencephalon radial glial cell
IHC AB chemical treatment: LY-411575 Adult dorsal telencephalon radial glial cell
IHC notch3fh332/+ control Adult dorsal telencephalon radial glial cell
IHC notch3fh332/+ chemical treatment: LY-411575 Adult dorsal telencephalon radial glial cell
Ab1-s100 IHC AB control Adult dorsal telencephalon radial glial cell
IHC AB + MO6-notch3 standard conditions Adult dorsal telencephalon radial glial cell
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment: LY-411575 Adult radial glial cell division in pallium occurrence, normal
AB + MO6-notch3 standard conditions Adult dorsal telencephalon radial glial cell proliferative, abnormal
Adult radial glial cell division in pallium increased occurrence, abnormal
notch3fh332/+ control Adult radial glial cell division in pallium occurrence, normal
notch3fh332/+ chemical treatment: LY-411575 Adult dorsal telencephalon radial glial cell proliferative, abnormal
Adult radial glial cell division in pallium increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development