ZFIN ID: ZDB-FIG-130808-11
Hayes et al., 2013 - Expression of glycosaminoglycan epitopes during zebrafish skeletogenesis. Developmental dynamics : an official publication of the American Association of Anatomists   242(6):778-89 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Condition:
Anatomical Terms:
Stage Range: Day 4 to Days 7-13

Fig. 2

Various chondroitin sulfate moities are expressed in overlapping compartments during zebrafish skeletal development. A: Wholemount zebrafish heads labelled for various chondroitin and keratan sulfate epitopes with monoclonal antibodies 1B5, 2B6, 3B3, 7D4, and 5D4 (refer to Table 2, for antibody specificities). All images are confocal reconstructions presented with anterior facing to the left, and shown as ventral or lateral views. Scale bars = 100 μm in all panels. Triangular arrowheads in C-0-S and KS panels point to enrichment of GAG epitope in the jaw joint. Acute arrowheads in C-0-S panel point to expression in the lens. Acute arrowheads point to ossifying region of the ceratohyal. B: Chondroitinase ABC untreated controls show decreased/no immunoreactivity with the specific CS epitope antibodies. Treatment with chondroitinase ABC is required to generate the epitopes recognised by the 1B5 and 2B6 antibodies and, as such, chondroitinase untreated larvae show no immunoreactivity demonstrating that the antibodies are specific. The 3B3 antibody can also recognise an epitope present in native CS chains, so larvae untreated with chondroitinase ABC show changed rather than absent immunoreactivity with 3B3. mc, Meckel′s cartilage; ch, ceratohyal; ba, branchial arches; op, operculum; cl, cleithrum; 5ba, 5th branchial arch and teeth; nc, notochord; mx, maxilla; br, branchiosteal rays; hs, hyosymplectic; C′ase, chondroitinase.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-1B5 IHC WT control Day 4 Not Detected ceratohyal cartilage
IHC Day 4 Not Detected Meckel's cartilage
IHC WT standard conditions Day 4 ceratohyal cartilage
IHC Day 4 lens
IHC Day 4 Meckel's cartilage
IHC Day 4 quadrate-anguloarticular joint
IHC Days 7-13 branchiostegal ray
IHC Days 7-13 cleithrum
IHC Days 7-13 opercular flap
IHC Days 7-13 pharyngeal arch 2 skeleton
IHC Days 7-13 pharyngeal arch 5 skeleton
IHC WT chemical treatment: pharmaceutical Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
Ab1-2B6 IHC WT control Day 4 Not Detected ceratohyal cartilage
IHC Day 4 Not Detected Meckel's cartilage
IHC WT standard conditions Day 4 ceratohyal cartilage
IHC Day 4 hyosymplectic cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 pharyngeal arch 3-7
IHC Days 7-13 opercular flap
IHC Days 7-13 pharyngeal arch 2 skeleton
IHC WT chemical treatment: pharmaceutical Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
Ab1-3B3+ IHC WT control Day 4 Not Detected ceratohyal cartilage
IHC Day 4 Not Detected Meckel's cartilage
IHC WT standard conditions Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 pharyngeal arch 3-7
IHC Days 7-13 branchiostegal ray
IHC Days 7-13 cleithrum
IHC Days 7-13 hyosymplectic cartilage
IHC Days 7-13 opercular flap
IHC Days 7-13 pharyngeal arch 2 skeleton
IHC Days 7-13 pharyngeal arch 5 skeleton
IHC WT chemical treatment: pharmaceutical Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
Ab1-5D4 IHC WT standard conditions Day 4 ceratohyal cartilage
IHC Day 4 hyosymplectic cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 quadrate-anguloarticular joint
IHC Days 7-13 branchiostegal ray
IHC Days 7-13 ceratohyal cartilage
IHC Days 7-13 cleithrum
IHC Days 7-13 notochord
IHC Days 7-13 opercular flap
IHC Days 7-13 pharyngeal arch 2 skeleton
IHC Days 7-13 pharyngeal arch 5 skeleton
Ab1-7D4 IHC WT standard conditions Day 4 ceratohyal cartilage
IHC Day 4 hyosymplectic cartilage
IHC Day 4 Meckel's cartilage
IHC Days 7-13 ceratohyal cartilage
IHC Days 7-13 hyosymplectic cartilage
IHC Days 7-13 Meckel's cartilage
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.