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ZIRC
ZFIN ID: ZDB-FIG-130524-16
Chou et al., 2013 - Fibronectin mediates correct positioning of the interrenal organ in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   242(5):432-443 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Pec-fin
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Pec-fin

Fig. 3 The effect of ets1bMO on the Fn distribution in the interrenal region. Top: Transverse sections of Tg(kdrl:GFP)s843 embryos either uninjected (A, A′) or injected with ets1bMO (B, B′) were harvested at 34 hpf and assayed for 3β-Hsd activity (black), GFP (green), and Fn expression (red in A, B; white in A′, B′). The result of ets1b morphant in B, B′ is a representative of 9 sectioned samples, which all showed disrupted vasculature at the level of the midtrunk. Bottom: Transverse sections of Tg(ff1bEx2:GFP) embryos either uninjected (C, C′) or injected with ets1bMO (D, D′) were harvested at 60 hpf and assayed for GFP (green), Fn expression (red), and pFAK (blue). C′, D′ are magnified views of the outlined areas in C, D. The result of ets1b morphant in D, D′ is a representative of 20 sectioned samples. The accumulation of Fn in the interrenal microenvironment is severely reduced in the ets1b morphant. All sections are oriented with the dorsal side toward the top. DA, dorsal aorta; IR, interrenal tissue; NC, notochord; NT, neural tube; S, somite; SB, swim bladder.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP s843Tg standard conditions Prim-15 blood vessel IFL
Prim-15 dorsal aorta IFL
Pec-fin interrenal primordium IFL
s843Tg + MO1-etv2 + MO2-etv2 standard conditions Prim-15 blood vessel IFL
Pec-fin interrenal primordium IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-ptk2 IHC zf346Tg control Pec-fin interrenal primordium
IHC Pec-fin intestine
IHC Pec-fin swim bladder
IHC zf346Tg + MO1-etv2 + MO2-etv2 standard conditions Pec-fin intestine
IHC Pec-fin swim bladder
Ab2-fn IHC s843Tg standard conditions Prim-15 notochord
IHC Prim-15 presumptive swim bladder
IHC Prim-15 somite
IHC s843Tg + MO1-etv2 + MO2-etv2 standard conditions Prim-15 notochord
IHC Prim-15 presumptive swim bladder
IHC Prim-15 somite
IHC zf346Tg control Pec-fin dorsal aorta
IHC Pec-fin intestine
IHC Pec-fin swim bladder
IHC zf346Tg + MO1-etv2 + MO2-etv2 standard conditions Pec-fin intestine
IHC Pec-fin swim bladder
Phenotype Details
Fish Conditions Stage Phenotype
s843Tg + MO1-etv2 + MO2-etv2 standard conditions Prim-15 blood vessel development disrupted, abnormal
Prim-15 dorsal aorta absent, abnormal
zf346Tg + MO1-etv2 + MO2-etv2 standard conditions Pec-fin blood vessel development disrupted, abnormal
Pec-fin dorsal aorta absent, abnormal
Pec-fin intestine decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.