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ZFIN ID: ZDB-FIG-130503-45
Sheehan-Rooney et al., 2013 - Bmp and Shh Signaling Mediate the Expression of satb2 in the Pharyngeal Arches. PLoS One   8(3):e59533 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec

Fig. 1

Expression of satb2 requires Hh and Bmp signaling.

(A–L) Lateral images of satb2 expression at 36 or 48 hpf. (A–D) Compared to wild-type embryos, in 36 and 48 hpf shha embryos, satb2 expression is reduced in the palatal precursors (arrows in C and D) and throughout the pharyngeal arches. (E,F) Nearly all neural crest cell expression of satb2 appears to be absent in smo embryos. The prominent expression in panel F, is in neural tissue. (G,H) Weak to no expression of satb2 was observed in smad5 mutant embryos at 36 and 48 hpf. (I,J) satb2 expression in edn1 mutants was similar wild-type embryos at both time points analyzed. (K,L) While expression of satb2 was absent throughout the posterior arches of hand2 mutants, the ventral first arch and palatal precursors maintained satb2 expression. p, palatal precursors; e, eye; The arches are numbered in A & C for clarity.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
satb2 AB standard conditions Prim-25 palate ISH
Prim-25 pharyngeal arch ISH
Long-pec palate ISH
Long-pec pharyngeal arch ISH
Df(Chr01:hand2)s6/s6 standard conditions Prim-25 palate ISH
Prim-25 pharyngeal arch 1 ISH
Long-pec palate ISH
Long-pec pharyngeal arch 1 ISH
edn1tf216b/tf216b standard conditions Prim-25 palate ISH
Prim-25 pharyngeal arch ISH
Long-pec palate ISH
Long-pec pharyngeal arch ISH
shhaDf(Chr07)t4/t4 standard conditions Prim-25 palate ISH
Prim-25 pharyngeal arch ISH
Long-pec palate ISH
Long-pec pharyngeal arch ISH
smad5b1100/b1100 standard conditions Prim-25 palate ISH
Prim-25 pharyngeal arch ISH
Long-pec Not Detected palate ISH
Long-pec pharyngeal arch ISH
smob577/b577; y1Tg standard conditions Prim-25 Not Detected palate ISH
Prim-25 Not Detected pharyngeal arch ISH
Long-pec Not Detected palate ISH
Long-pec Not Detected pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One