ZFIN ID: ZDB-FIG-130128-42
Garnaas et al., 2012 - Rargb regulates organ laterality in a zebrafish model of right atrial isomerism. Developmental Biology   372(2):178-189 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 2 Raldhs 2 and 4 temporally regulate liver development. (A–I) Wild type embryos were injected with raldh2 or raldh4 MO at the 1-cell stage, and liver development was assessed over the next 72 h. In situ hybridization for the hepatoblast marker prox1 at 30 hpf (A–C) and 48 hpf (D–F) and hepatocyte marker lfabp at 72 hpf (G–I) demonstrates distinct temporal requirements for Raldh2 and Raldh4 during liver development. Arrowheads denote the liver primordium, and the dotted lines outline the liver. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fabp10a WT control Protruding-mouth liver ISH
WT + MO2-aldh8a1 standard conditions Protruding-mouth liver ISH
WT + MO3-aldh1a2 standard conditions Protruding-mouth liver ISH
prox1a WT control Prim-15 liver ISH
Long-pec liver ISH
WT + MO2-aldh8a1 standard conditions Prim-15 liver ISH
Long-pec liver ISH
WT + MO3-aldh1a2 standard conditions Prim-15 liver ISH
Long-pec liver ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-aldh8a1 standard conditions Long-pec - Protruding-mouth liver decreased size, abnormal
WT + MO3-aldh1a2 standard conditions Prim-15 - Protruding-mouth liver decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 372(2), Garnaas, M.K., Cutting, C.C., Meyers, A., Kelsey, P.B., Harris, J.M., North, T.E., and Goessling, W., Rargb regulates organ laterality in a zebrafish model of right atrial isomerism, 178-189, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.