FIGURE

Fig. 4

ID
ZDB-FIG-130115-27
Publication
Sakowski et al., 2012 - Neuromuscular effects of G93A-SOD1 expression in zebrafish
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Fig. 4

Neuromuscular junction integrity in transgenic ALS zebrafish. NMJs in transverse muscle sections from control AB and transgenic G93A-SOD1-GFP zebrafish were examined at multiple timepoints after αBTX staining (red) to label AChR clusters in the muscle fibers and SV2/NF IHC (green) to label MNs. Colocalization (yellow) is apparent at intact NMJs. (A-B) Representative control AB zebrafish (A) exhibit long axons with multiple synapses, whereas transgenic G93A-SOD1-GFP zebrafish (B) exhibit shorter axons and varied innervation patterns with some denervated NMJs (arrowheads). 60x oil magnification confocal images; scale bar = 20 μm. (C-D) A representative image of an individual neuron, with images included as both separated and merged fluorescent channels (C) and visualized with orthogonal XZ and YZ views of the z-series confocal image (D), validates that colocalization of SV2/NF and αBTX is representative of NMJs. 60x oil magnification confocal images; scale bar = 20 μm. (E) Quantification of SV2/NF:αBTX colocalization in 10-60 week old control AB and transgenic G93A-SOD1-GFP zebrafish. * P < 0.0001 compared to age-matched AB controls.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Mol. Neurodegener.