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ZIRC
ZFIN ID: ZDB-FIG-121217-34
Nguyen-Chi et al., 2012 - Morphogenesis and Cell Fate Determination within the Adaxial Cell Equivalence Group of the Zebrafish Myotome. PLoS Genetics   8(10):e1003014 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Pec-fin
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 7 Radar or BMP signaling knock-down impairs MP formation.

(A) The level of expression of spliced radar and β-actin mRNAs was analysed by semi-quantitative RT-PCR. Total RNA extracts were prepared at 1 dpf from uninjected embryos and from embryos that were injected with morpholinos blocking the splicing of radar (rdrMO). The number of cycles is shown. (B) Graphic representation of the number of MPs per somite at 1 dpf in the indicated conditions, values = mean, error bars = s.e.m., ***p<0.001. (C–D) eng2a expression at the 12-somite stage in (C) uninjected, or (D) rdrMO-injected embryos, as determined by ISH. (E–J) MPs staining with anti-Eng (green) and anti-Prox1 (red) antibodies at 1 dpf embryos in (E) uninjected, (F) rdrMO-injected embryos, (G) p53MO- and rdrMO-injected embryos, (H) radar mutant (rdrs327), (I) Dorsomorphin (DM) treated embryos and (J) SU5402-treated embryos that were injected with rdrMO, scale bar = 20 μm. (K–M) sMyhc (red) expression in the somites of (K) WT, (L) radar morphans and (M) P53/radar double morphans (rdrMO/P53MO) at 1 dpf, 3D reconstructions of multiple confocal scans, lateral and cross section views, scale bar = 20 μm. (N–Q) Eng2a expression in the somites of 1 dpf embryos after treatment with increasing doses of Dorsomorphin (10, 20 and 50 μM) or with DMSO (1%), scale bar 100 μm. (R–S) GFP expression in Tg(5XBRE[vent2]:-20lid3:GFP) embryos at 60 hpf after (S) DM (50 μM) or (R) DMSO (1%) treatments, showing that DM treatment inhibits BMP signaling. (T–W) Eng2a expression in 1 dpf embryos that were (U–W) injected or (T–V) not with rdrMO and treated with either (T–U) DMSO or (V–W) SU5402.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP ir1189Tg standard conditions Pec-fin somite IHC
ir1189Tg chemical treatment: pharmaceutical Pec-fin Not Detected somite IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-F59 IHC WT standard conditions Prim-5 migratory slow muscle precursor cell
IHC WT + MO1-gdf6a standard conditions Prim-5 migratory slow muscle precursor cell
IHC WT + MO1-gdf6a + MO4-tp53 standard conditions Prim-5 migratory slow muscle precursor cell
Ab1-eng IHC WT standard conditions 10-13 somites muscle pioneer
IHC Prim-5 muscle pioneer
IHC WT chemical treatment: pharmaceutical Prim-5 muscle pioneer
IHC WT chemical treatment: SU5402 Prim-5 muscle pioneer
IHC WT + MO1-gdf6a chemical treatment: SU5402 Prim-5 muscle pioneer
IHC WT + MO1-gdf6a standard conditions 10-13 somites muscle pioneer
IHC Prim-5 muscle pioneer
IHC WT + MO1-gdf6a + MO4-tp53 standard conditions Prim-5 muscle pioneer
IHC gdf6as327/s327 standard conditions Prim-5 muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical Prim-5 muscle pioneer increased amount, abnormal
WT + MO1-gdf6a chemical treatment: SU5402 Prim-5 muscle pioneer increased amount, abnormal
WT + MO1-gdf6a standard conditions Prim-5 muscle pioneer increased amount, abnormal
WT + MO1-gdf6a + MO4-tp53 standard conditions Prim-5 muscle pioneer increased amount, abnormal
gdf6as327/s327 standard conditions Prim-5 muscle pioneer increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.