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Fig. 7

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ZDB-IMAGE-121217-13
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Figures for Nguyen-Chi et al., 2012
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Fig. 7 Radar or BMP signaling knock-down impairs MP formation.

(A) The level of expression of spliced radar and β-actin mRNAs was analysed by semi-quantitative RT-PCR. Total RNA extracts were prepared at 1 dpf from uninjected embryos and from embryos that were injected with morpholinos blocking the splicing of radar (rdrMO). The number of cycles is shown. (B) Graphic representation of the number of MPs per somite at 1 dpf in the indicated conditions, values = mean, error bars = s.e.m., ***p<0.001. (C–D) eng2a expression at the 12-somite stage in (C) uninjected, or (D) rdrMO-injected embryos, as determined by ISH. (E–J) MPs staining with anti-Eng (green) and anti-Prox1 (red) antibodies at 1 dpf embryos in (E) uninjected, (F) rdrMO-injected embryos, (G) p53MO- and rdrMO-injected embryos, (H) radar mutant (rdrs327), (I) Dorsomorphin (DM) treated embryos and (J) SU5402-treated embryos that were injected with rdrMO, scale bar = 20 μm. (K–M) sMyhc (red) expression in the somites of (K) WT, (L) radar morphans and (M) P53/radar double morphans (rdrMO/P53MO) at 1 dpf, 3D reconstructions of multiple confocal scans, lateral and cross section views, scale bar = 20 μm. (N–Q) Eng2a expression in the somites of 1 dpf embryos after treatment with increasing doses of Dorsomorphin (10, 20 and 50 μM) or with DMSO (1%), scale bar 100 μm. (R–S) GFP expression in Tg(5XBRE[vent2]:-20lid3:GFP) embryos at 60 hpf after (S) DM (50 μM) or (R) DMSO (1%) treatments, showing that DM treatment inhibits BMP signaling. (T–W) Eng2a expression in 1 dpf embryos that were (U–W) injected or (T–V) not with rdrMO and treated with either (T–U) DMSO or (V–W) SU5402.

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