Fig. S4

Radar/gdf6a morpholinos-induced phenotype is reversed by WT rdr mRNA injection. (A-E) Live embryos at 1 dpf either (A) uninjected or (B) injected with rdr morpholinos (rdr^MO) showing reduced eyes, massive cell death in the anterior neural tube and flatten somites (arrow heads, C1 phenotype). (C-D) Injections of 10–100 pg of rdr mRNA either alone (not shown) or in combination with rdr^MO induce severe gastrulation defects as described in Sidi et al, 2003 (C2 and C3 phenotype). (E) Only a low quantity of rdr mRNA (0.5–1 pg) rescue the phenotype induced by rdr^MO injection (C4 phenotype or WT). (F) Graphic representation of the percentage of embryos exhibiting WT, C1-C4 or dead phenotype in indicated conditions. (G-I) Prox1 (red) and Engrailed (green) expression in the somites of 1 dpf embryos either (G) uninjected or injected with (H) rdr^MO alone or (I) in combination with 1 pg of rdr mRNA. Increased MP number observed in rdr^MO injected embryos is reversed by WT rdr mRNA, showing that altered MP number is specifically induced by rdr loss-of-function.