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ZFIN ID: ZDB-FIG-121203-8
Chen et al., 2012 - MANF regulates dopaminergic neuron development in larval zebrafish. Developmental Biology   370(2):237-249 Full text @ Dev. Biol.
Anatomical Terms:
Stage: Adult

Fig. 2 In situ hybridization mRNA analysis and immunocytochemistry of MANF expression in adult zebrafish brain. (A) A sagittal view shows manf mRNA in an adult brain section. Expression can be seen in ventral telencephalic, preoptic, ventral thalamic, pretectal, dorsal thalamic, and hypothalamic regions. (B) A sagittal view of the brain shows immuoreactivity for MANF protein. Expression is shown in telencephalic, preoptic, thalamic, optic tectal, cerebellar and rhombencephalic ventricular regions. MANF antibody co-immunostaining with HuC/D antibody in ventral telencephalic (C), dorsal thalamic (D), and rhombencephalic ventricular regions (E). Co-immunostaining with zrf-1 in dorsal telencephalon (F), ventral thalamic region (G), and telencephalic ventricle (H). Co-immunostaining with TH1 in ventral thalamic area (I), preoptic complex (J), and in caudal hypothalamus (K). MANF staining is shown in green; HuC/D, zrf-1 and TH staining are shown in red. Cce: cerebellar corpus. Ha: habenula. Hv: ventral zone of periventricular hypothalamus. OB: olfactory bulb. PPa: parvocellular preoptic nucleus, anterior part. T: thalamus. TeO: tectum opticum. TeV: tectal ventricle. Vv: ventral nucleus of ventral telencephalic area. Scale bar=300 μm in (B), 100 μm in (K).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
manf Ab1-manf WT standard conditions Adult cerebellum IHC
Ab1-manf Adult dorsal telencephalon IHC
Adult dorsal thalamus ISH
Ab1-manf Adult dorsal thalamus IHC
Ab1-manf Adult fourth ventricle IHC
Adult hypothalamus ISH
Ab1-manf Adult hypothalamus posterior region IHC
Ab1-manf Adult optic tectum IHC
Adult preoptic area ISH
Ab1-manf Adult preoptic area IHC
Adult pretectum ISH
Ab1-manf Adult telencephalic ventricle IHC
Ab1-manf Adult telencephalon IHC
Ab1-manf Adult thalamus IHC
Adult ventral telencephalon ISH
Ab1-manf Adult ventral telencephalon IHC
Ab1-manf Adult ventral thalamus IHC
Adult ventral thalamus ISH
th Ab3-th WT standard conditions Adult hypothalamus posterior region IHC
Ab3-th Adult preoptic area IHC
Ab3-th Adult ventral thalamus IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT standard conditions Adult dorsal thalamus
IHC Adult fourth ventricle
IHC Adult ventral telencephalon
zrf-1 IHC WT standard conditions Adult dorsal telencephalon
IHC Adult telencephalic ventricle
IHC Adult ventral thalamus
Phenotype Details No data available
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 370(2), Chen, Y.C., Sundvik, M., Rozov, S., Priyadarshini, M., and Panula, P., MANF regulates dopaminergic neuron development in larval zebrafish, 237-249, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.