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ZFIN ID: ZDB-FIG-120810-45
Feng et al., 2012 - Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE(2). Current biology : CB   22(13):1253-1259 Full text @ Curr. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 3

Leukocyte Depletion Prevents V12RAS+ Cell Growth, which Can Be Partially Rescued by dmPGE2(A–I) Representative images of flank skin regions illustrating numbers of V12RASeGFP+ transformed cells after morpholino (Mo) knockdown of pu.1 and gcsfr (for depletion of both neutrophils and macrophages); gcsfr alone (for depletion of neutrophils); irf8 or a lower dose (2 nl/embryo) of pu.1 morpholino alone (for depletion of macrophages) with or without additional treatment with dmPGE2.(J) Graphic illustration of how dmPGE2 can rescue the number of transformed cells in morphants in which both neutrophils and macrophages have been specifically depleted (p < 0.01, n = 33, 32, and 34).(K) Graphic illustration of how dmPGE2 can only partially rescue morpholino knockdowns that largely target neutrophils (p < 0.001, n = 25, 25, and 22).(L and M) Similar graphic illustration of how dmPGE2 can rescue morpholino knockdowns that largely target macrophages (irf8 Mo-p < 0.05, n = 24, 26, and 23; 2 nl pu.1 Mo-p < 0.05, n = 31, 30, and 34).The scale bar represents 100 µm. See also Figures S3 and S4.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hzm1Et; io006Tg + MO1-irf8 chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil increased amount, abnormal
hzm1Et; io006Tg + MO1-irf8 standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil increased amount, abnormal
hzm1Et; io006Tg + MO1-spi1b chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
hzm1Et; io006Tg + MO1-spi1b standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r chemical treatment: prostaglandin E2 Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil decreased amount, abnormal
hzm1Et; io006Tg + MO1-spi1b + MO3-csf3r standard conditions Day 4 macrophage decreased amount, abnormal
Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil decreased amount, abnormal
hzm1Et; io006Tg + MO3-csf3r chemical treatment: prostaglandin E2 Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil decreased amount, abnormal
hzm1Et; io006Tg + MO3-csf3r standard conditions Day 4 mucus secreting cell decreased amount, abnormal
Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.