ZFIN ID: ZDB-FIG-120810-46
Feng et al., 2012 - Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE(2). Current biology : CB   22(13):1253-1259 Full text @ Curr. Biol.
Anatomical Term:
Stage: Day 4
Observed In:
Stage: Day 4

Fig. 4 Live Imaging Reveals that Reduced PGE2 Levels as a Result of COX-2 Inhibition Lead to Leukocyte Behavior Change and Increased Transformed-Cell Death(A and B) Still images from movies illustrating neutrophil (red) numbers and their motility in the vicinity of V12RAS transformed cells (green) in (A) control and (B) NS398-treated larvae (images are taken from Movie S1) (A′ and B′) show the tracks over 100 min of neutrophil movements from the two movies S1A and S1B, respectively, revealing reduced neutrophil motility in NS398-treated (B2) compared with control (A′) larva. Scale bars represent 50 μm.(C) Quantification of neutrophil migration velocity (V = mean ± SD); DMSO-treated V = 0.125 ± 0.046 μm (n = 39); NS398-treated V = 0.054 ± 0.038 μm (n = 32).(D) A series of still images from a time-lapse movie (see Movie S2) showing transformed-cell death associated with a macrophage encounter in a larva treated with NS398; subsequently, this macrophage and others engulf the resulting cell debris. Red arrows in (i)–(iv) indicate a macrophage labeled by NFκB-RE:eGFP (pale green). Purple arrow in (v) indicates a macrophage engulfing the transformed-cell debris and forming a phagosome inside its cell body. The scale bar represents 15 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP hzm1Et; io006Tg; nz50Tg; sh235Tg chemical treatment: pharmaceutical Day 4 macrophage IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hzm1Et; io006Tg; nz50Tg chemical treatment: pharmaceutical Day 4 mucus secreting cell proliferative, abnormal
Day 4 neutrophil decreased cellular motility, abnormal
hzm1Et; io006Tg; nz50Tg; sh235Tg chemical treatment: pharmaceutical Day 4 cell death increased occurrence, abnormal
Day 4 mucus secreting cell proliferative, abnormal
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.