ZFIN ID: ZDB-FIG-120810-41
Feng et al., 2012 - Live Imaging of Tumor Initiation in Zebrafish Larvae Reveals a Trophic Role for Leukocyte-Derived PGE(2). Current biology : CB   22(13):1253-1259 Full text @ Curr. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. S3 Morpholinos are still effective throughout the timecourse of our transformed cell number analysis (related to Figures 1 and 3)

(A) RT-PCR results showing that mPGES morpholino is still capable of significant knockdown of wild type mPGES mRNA until at least 5dpf. (B-E) Wide field images overlayed with red fluorescent views of Tg (lysC:DsRed) larvae, showing neutrophils still suppressed in pu.1+gcsfr Morpholino and gcsfr Morpholino injected larvae at 4.5dpf. Scale bar=150μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nz50Tg + MO1-spi1b + MO3-csf3r standard conditions Day 4 neutrophil decreased amount, abnormal
nz50Tg + MO3-csf3r standard conditions Day 4 neutrophil decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Current biology : CB for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Curr. Biol.