ZFIN ID: ZDB-FIG-120315-65
Schaub et al., 2012 - Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network. Developmental Biology   363(1):128-137 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1

Morphological and physiological phenotypes of s445 mutant larvae at 120 hpf. (A and B) Bright-field images of wild-type (A) and s445 mutant (B) larvae at 120 hpf are overlaid with Tg(gutGFP)s854 expression (green). The physiological appearance of s445 mutant larvae is normal, however their liver size is smaller at 120 hpf. Lateral views, anterior to the left. White arrowheads point to the liver. (C and D) Fluorescent image of wild-type (C) and s445 mutant (D) Tg(fabp1a:DsRed)gz15 larvae at 120 hpf. Liver size visualized by Tg(fabp1a:DsRed)gz15 expression is smaller in s445 mutant larvae. Lateral views, anterior to the left. (E and F) Fluorescent images of wild-type (E) and s445 mutant (F) larvae soaked in PED6. PED6 is processed by phospholipase and fluorescence is detected in the intestine in both wild-type and s445 mutant larvae. However, PED6 fluorescence is missing from the s445 mutant gallbladder. Lateral views, anterior to the right. Asterisks indicate the intestine. (G and H) Projected confocal image of the gallbladders of wild-type (G) and s445 mutant (H) Tg(gutGFP)s854 larvae. The gallbladder and the extrahepatic bile duct visualized by Tg(gutGFP)s854 expression are schematically presented in (G2 and H2). Ventral views, anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed gz15Tg standard conditions Day 5 liver IFL
snapc4s445/s445; gz15Tg standard conditions Day 5 liver IFL
GFP s854Tg standard conditions Day 5 extrahepatic duct IFL
Day 5 gall bladder IFL
Day 5 gut IFL
Day 5 liver IFL
snapc4s445/s445; s854Tg standard conditions Day 5 extrahepatic duct IFL
Day 5 gall bladder IFL
Day 5 gut IFL
Day 5 liver IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
snapc4s445/s445; gz15Tg standard conditions Day 5 liver decreased size, abnormal
snapc4s445/s445; s854Tg standard conditions Day 5 liver and biliary system decreased functionality, abnormal
Day 5 liver decreased size, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 363(1), Schaub, M., Nussbaum, J., Verkade, H., Ober, E.A., Stainier, D.Y., and Sakaguchi, T.F., Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network, 128-137, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.