ZFIN ID: ZDB-FIG-120315-73
Schaub et al., 2012 - Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network. Developmental Biology   363(1):128-137 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. S3

Phenocopy of the s445 mutant phenotype by morpholino antisense oligonucleotides. (A–F) Lateral views of wild-type control (A and B), snapc4 MO(A) injected (C and D), and snapc4 MO(B) injected (E and F) larvae at 120 hpf. Tg(ela3l:EGFP)gz15 expression is shown in B, D, and F. snapc4 MO(A) injected larvae show an identical phenotype to that of s445 mutant larvae. Lateral views, anterior to the left (A, C and E) or the right (B, D and F). (G) Schematic of snapc4 morpholino target locations. MO(A) is designed to target the splice junction prior to the Snapc2 binding domain. MO(B) is designed to target the splice junction prior to the Myb DNA binding domain. MO(C) is designed to target the translation initiation site. (H) Reverse transcriptase-PCR analysis shows that snapc4 mRNA was truncated in snapc4 MO(B) injected larvae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP gz15Tg control Day 5 gut IFL
gz15Tg + MO1-snapc4 standard conditions Day 5 gut IFL
gz15Tg + MO2-snapc4 standard conditions Day 5 gut IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-snapc4 standard conditions Day 5 liver and biliary system decreased functionality, abnormal
Day 5 liver decreased size, abnormal
WT + MO2-snapc4 standard conditions Day 5 liver and biliary system decreased functionality, abnormal
Day 5 liver decreased size, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 363(1), Schaub, M., Nussbaum, J., Verkade, H., Ober, E.A., Stainier, D.Y., and Sakaguchi, T.F., Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network, 128-137, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.