ZFIN ID: ZDB-FIG-120126-2
Peukert et al., 2011 - Lhx2 and lhx9 determine neuronal differentiation and compartition in the caudal forebrain by regulating wnt signaling. PLoS Biology   9(12):e1001218 Full text @ PLoS Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 2

Differentiation of thalamic neurons is stalled in lhx2/lhx9 morphant embryos.

Analysis of embryos for neuronal differentiation in double morphant embryos at 48 hpf, lateral view (a, b, c, etc.), and cross-section of left hemispheres (a2, b2, c2 etc.) of the same embryo are shown. The expression domain of the neuronal precursor deltaA at the ventricular zone (VZ) is vigorously expanded in double morphant embryos (a–b2). Expression of the progenitor marker neurog1 marking the subventricular zone (SVZ, white bars) is also broadened in lhx2/lhx9 morphant embryos compared to control embryos (c–d2). However, the thalamus-specific terminal differentiation markers, id2a and lef1, are down-regulated in the mantle zone of the cTh (MZ, black bars) of Lhx2/Lhx9-deficient embryos (e–h2). The postmitotic marker gbx2 shows no alteration in the compound morphant embryos (i–j2). The number of cells expressing the pan-postmitotic neuronal marker elavl3 is strongly decreased in the double morphant embryos shown by a confocal microscope section of an transgenic Elavl3:GFP transgenic embryos (k, l). The deltaA and neurog1 positive precursor pool in the ventricular/subventricular zone (blue and green domain) expands on the expense of the post-mitotic thalamic neurons (red domain) in the mantle zone in lhx2/lhx9 morphant embryos (m, n). ETh, epithalamus; HyTh, hypothalamus; MDO, mid-diencephalic organizer; MZ, mantle zone; pTu, posterior tuberculum; VZ, ventricular zone.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dla WT control Long-pec dorsal thalamus ventricular zone ISH
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus ventricular zone ISH
EGFP knu3Tg control Long-pec dorsal thalamus neuron IFL
knu3Tg + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus neuron IFL
gbx2 WT control Long-pec dorsal thalamus posterior region ISH
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus posterior region ISH
id2a WT control Long-pec dorsal thalamus ISH
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus ISH
lef1 WT control Long-pec dorsal thalamus ISH
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus ISH
neurog1 WT control Long-pec dorsal thalamus ISH
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-lhx9 + MO3-lhx2b standard conditions Long-pec dorsal thalamus CNS neuron (sensu Vertebrata) poorly differentiated, abnormal
Long-pec forebrain neuron differentiation decreased process quality, abnormal
Long-pec thalamus development decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Biol.