ZFIN ID: ZDB-FIG-111219-5
Yang et al., 2011 - Twist controls skeletal development and dorsoventral patterning by regulating runx2 in zebrafish. PLoS One   6(11):e27324 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Term:
Stage: Protruding-mouth

Fig. 3

Hypoxia increases the expression of hif1-α and twist in zebrafish.

Zebrafish at 48 hpf were treated with or without 21% O2, 5% O2 or indicated concentration of DFX for 1 day and the expression of hif-1α, twist1a, twist1b, twist2 and twist3 genes was assayed by quantitative RT-PCR. Results are shown as the relative expression to ²-actin (mean ± SD) and significance was determined by Student′s t-test. (* p<0.05 and ** p<0.01 versus 21% O2).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
twist1a WT control Protruding-mouth whole organism RTPCR
WT chemical treatment: pharmaceutical Protruding-mouth whole organism RTPCR
WT hypoxia Protruding-mouth whole organism RTPCR
twist1b WT control Protruding-mouth whole organism RTPCR
WT chemical treatment: pharmaceutical Protruding-mouth whole organism RTPCR
WT hypoxia Protruding-mouth whole organism RTPCR
twist2 WT control Protruding-mouth whole organism RTPCR
WT chemical treatment: pharmaceutical Protruding-mouth whole organism RTPCR
WT hypoxia Protruding-mouth whole organism RTPCR
twist3 WT control Protruding-mouth whole organism RTPCR
WT chemical treatment: pharmaceutical Protruding-mouth whole organism RTPCR
WT hypoxia Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One