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ZFIN ID: ZDB-FIG-110928-27
Culbertson et al., 2011 - Chondrogenic and Gliogenic Subpopulations of Neural Crest Play Distinct Roles during the Assembly of Epibranchial Ganglia. PLoS One   6(9):e24443 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 2

Disruptions in neural crest cause late defects in epibranchial ganglion morphology without affecting placode specification.

(A–E) Ventral view of head skeleton visualized with anti-Collagen2a antibody at 4 dpf. (A) The following structures are visible in wildtype: Meckel′s cartilage, palatoquadrate, ceratohyal and ceratobranchial arches. Sox10 mutant (B) exhibits normal morphology. Radical disruption of structure is apparent in foxd3 mutants (C) and disc1 morphants (E). prdm1a mutant (D) displays grossly normal anterior jaw structures and deficits in the posterior arches; in this example, an unpaired ceratobranchial vestige can be seen (arrowhead). (F–J) Anti-Pax2 immunofluorescence at 24 hpf. Wildtype (F) shows facial placode and unsegmented glossopharyngeal/vagal placode field (K–O) phox2b expression at 48 hpf. Wildtype (K) displays normal segregation of facial,glossopharyngeal, and vagal ganglia. Defects are apparent in foxd3 (M) and prdm1a (N) mutants and disc1 morphant (O). sox10 mutant (K) display morphology and segmentation comparable to wildtype siblings (F). P–T: Ganglion morphology visualized by anti-Elavl3/4 antibody staining at 4 dpf. (K) Wildtype control shows glossopharyngeal and vagal ganglia. Posterior lateral line, statoacoustic and the fused trigeminal, anterior lateral line and facial ganglia can also be seen. Defects are apparent in sox10 (Q), foxd3 (R), prdm1a (S) mutants and disc1 morphant (T). Arrow in (S) shows an example of fusion often seen in prdm1a mutants. Abbreviations: mk = Meckel′s cartilage; pq = palatoquadrate; ch = ceratohyal; cb = ceratobranchial arches; OV = otic vesicle; F = facial ganglion; Tg/aLL/F = trigeminal/anterior lateral line/facial ganglion complex; SA = statoacoustic ganglion; pLLg = posterior lateral line ganglion; G = glossopharyngeal ganglion; V = vagal ganglia. Scale bar in (A) = 100 μm. Scale bars in (F), (K) and (P) = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
phox2bb WT standard conditions Long-pec epibranchial ganglion ISH
Long-pec facial ganglion ISH
Long-pec glossopharyngeal ganglion ISH
Long-pec vagal ganglion ISH
WT + MO3-disc1 standard conditions Long-pec epibranchial ganglion ISH
foxd3zdf10/zdf10 standard conditions Long-pec epibranchial ganglion ISH
prdm1anl3/nl3 standard conditions Long-pec epibranchial ganglion ISH
sox10t3/t3 standard conditions Long-pec epibranchial ganglion ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-col2a IHC WT standard conditions Day 4 ceratobranchial cartilage
IHC Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 palatoquadrate cartilage
IHC WT + MO3-disc1 standard conditions Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 palatoquadrate cartilage
IHC foxd3zdf10/zdf10 standard conditions Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 palatoquadrate cartilage
IHC prdm1anl3/nl3 standard conditions Day 4 ceratobranchial cartilage
IHC Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 palatoquadrate cartilage
IHC sox10t3/t3 standard conditions Day 4 ceratobranchial cartilage
IHC Day 4 ceratohyal cartilage
IHC Day 4 Meckel's cartilage
IHC Day 4 palatoquadrate cartilage
Ab1-elavl IHC WT standard conditions Day 4 anterior lateral line ganglion
IHC Day 4 facial ganglion
IHC Day 4 glossopharyngeal ganglion
IHC Day 4 posterior lateral line ganglion
IHC Day 4 statoacoustic (VIII) ganglion
IHC Day 4 trigeminal ganglion
IHC Day 4 vagal ganglion
IHC WT + MO3-disc1 standard conditions Day 4 epibranchial ganglion
IHC foxd3zdf10/zdf10 standard conditions Day 4 epibranchial ganglion
IHC prdm1anl3/nl3 standard conditions Day 4 epibranchial ganglion
IHC sox10t3/t3 standard conditions Day 4 epibranchial ganglion
Ab1-pax2 IHC WT standard conditions Prim-5 epibranchial placode
IHC Prim-5 facial placode
IHC Prim-5 otic vesicle
IHC WT + MO3-disc1 standard conditions Prim-5 epibranchial placode
IHC Prim-5 facial placode
IHC Prim-5 otic vesicle
IHC foxd3zdf10/zdf10 standard conditions Prim-5 epibranchial placode
IHC Prim-5 facial placode
IHC Prim-5 otic vesicle
IHC prdm1anl3/nl3 standard conditions Prim-5 epibranchial placode
IHC Prim-5 facial placode
IHC Prim-5 otic vesicle
IHC sox10t3/t3 standard conditions Prim-5 epibranchial placode
IHC Prim-5 facial placode
IHC Prim-5 otic vesicle
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-disc1 standard conditions Long-pec vagal ganglion malformed, abnormal
Day 4 cartilage morphogenesis decreased process quality, abnormal
Day 4 ceratobranchial cartilage absent, abnormal
Day 4 ceratohyal cartilage malformed, abnormal
Day 4 Meckel's cartilage malformed, abnormal
Day 4 palatoquadrate cartilage malformed, abnormal
Day 4 vagal ganglion malformed, abnormal
foxd3zdf10/zdf10 standard conditions Long-pec vagal ganglion malformed, abnormal
Day 4 cartilage morphogenesis decreased process quality, abnormal
Day 4 ceratobranchial cartilage absent, abnormal
Day 4 ceratobranchial cartilage malformed, abnormal
Day 4 ceratohyal cartilage malformed, abnormal
Day 4 vagal ganglion malformed, abnormal
prdm1anl3/nl3 standard conditions Long-pec vagal ganglion malformed, abnormal
Day 4 cartilage morphogenesis decreased process quality, abnormal
Day 4 ceratobranchial cartilage absent, abnormal
Day 4 ceratobranchial cartilage malformed, abnormal
Day 4 ceratohyal cartilage malformed, abnormal
Day 4 vagal ganglion malformed, abnormal
sox10t3/t3 standard conditions Long-pec ceratobranchial cartilage morphology, normal
sox10t3/t3 standard conditions Long-pec vagal ganglion morphology, normal
sox10t3/t3 standard conditions Day 4 vagal ganglion malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One