ZFIN ID: ZDB-FIG-110915-9
Zygmunt et al., 2011 - Semaphorin-PlexinD1 Signaling Limits Angiogenic Potential via the VEGF Decoy Receptor sFlt1. Developmental Cell   21(2):301-314 Full text @ Dev. Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 26+ somites to Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: 26+ somites

Fig. 1

Sema-PlxnD1 Signaling Is Cell Autonomously Required within the Endothelium for Proper SeA Sprout Abundance and Distribution

(A and B) 23 hpf vasculatures, green. SBs, red. Horizontal myoseptum (HM), white dotted line. SeA sprouts, numbered. (A) WT. (B) obd.

(C and D) SeA sprout position (C) and abundance (D) in 23 hpf WT and obd. n = 8 WT, 12 obd. Error bars represent SEM. ***p < 0.001.

(E and F) WISH, 28 hpf trunks. Ectopic SeA sprouts, white arrowheads. Riboprobes: flt4 (blue), cdh5 (red). WT (E). obd (F). n = 10/10 WT, 10/10 obd.

(G and H) 32 hpf chimeric vasculatures with ECs of donor (green) and host (red) origin. Examples of ectopic SeA sprouts, white arrowheads.

(A, B, E–H) Anterior, left; dorsal, up. Scale bars represent 30 μm. See Figure S1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh5 WT standard conditions Prim-5 endothelial cell ISH
plxnd1fov01b/fov01b standard conditions Prim-5 endothelial cell ISH
EGFP plxnd1fov01b/fov01b; y1Tg standard conditions 26+ somites vasculature IFL
y1Tg standard conditions 26+ somites vasculature IFL
flt4 WT standard conditions Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
plxnd1fov01b/fov01b standard conditions Prim-5 intersegmental vessel ISH
Prim-5 posterior cardinal vein ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
plxnd1fov01b/fov01b; y1Tg standard conditions 26+ somites angiogenic sprout increased amount, abnormal
26+ somites intersegmental vessel mislocalised, abnormal
26+ somites sprouting angiogenesis process quality, abnormal
Acknowledgments:
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Reprinted from Developmental Cell, 21(2), Zygmunt, T., Gay, C.M., Blondelle, J., Singh, M.K., Flaherty, K.M., Means, P.C., Herwig, L., Krudewig, A., Belting, H.G., Affolter, M., Epstein, J.A., and Torres-Vazquez, J., Semaphorin-PlexinD1 Signaling Limits Angiogenic Potential via the VEGF Decoy Receptor sFlt1, 301-314, Copyright (2011) with permission from Elsevier. Full text @ Dev. Cell