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ZFIN ID: ZDB-FIG-110816-5
Kabashi et al., 2011 - FUS and TARDBP but Not SOD1 Interact in Genetic Models of Amyotrophic Lateral Sclerosis. PLoS Genetics   7(8):e1002214 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 6

Mutant SOD1 exacerbates the motor phenotype caused by mutant FUS and mutant TARDBP.

A) Motor phenotype was assessed both by time frames obtained from video recordings of the TEER as well as B) immunohistochemical labeling of the axonal projections to determine UAL in motor neurons. The analysis demonstrates that knock-down of both Tardbp and Fus (ii) does not lead to an exacerbated phenotype when compared to phenotypes generated by KD of Tardbp (i) or Fus by AMOs. Similarly, overexpression of both mutant R521H FUS and G348C TARDBP (iv) does not aggravate the phenotype observed by overexpression of mutant TARDBP (iii) or mutant FUS (Figure 2A, 2Biv). However, co-expression of mutant SOD1 and mutant FUS did exacerbate the motor phenotype caused by mutant SOD1 alone (v). Arrowheads represent length to the first axonal branching (UAL). Scale bar: 40 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-SV2 IHC WT + MO1-fus + MO1-tardbp standard conditions Long-pec - Protruding-mouth motor neuron axon
IHC WT + MO1-tardbp standard conditions Long-pec - Protruding-mouth motor neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-fus + MO1-tardbp standard conditions Long-pec sensory perception of touch disrupted, abnormal
Long-pec - Protruding-mouth motor neuron axon decreased length, abnormal
WT + MO1-tardbp standard conditions Long-pec sensory perception of touch disrupted, abnormal
Long-pec - Protruding-mouth motor neuron axon decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.