ZFIN ID: ZDB-FIG-110804-81
Matsui et al., 2011 - Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis. Proceedings of the National Academy of Sciences of the United States of America   108(24):9881-6 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Shield to 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to 75%-epiboly

Fig. 5 cdh1 expression. (A and B) cdh1 (purple) and sox32 (red) expression in DFCcontrol-MO (A) or DFCcnpy1-MO (B) embryos at 65% epiboly stage. (C and D) tbx16 (purple) and sox32 (red) expression in DFCcontrol-MO (C) or DFCcnpy1-MO (D) embryos at 65% epiboly stage. Dotted lines in A–D mark the outlines of DFC populations. (Scale bar: 200 μm.) (E and F) Expression of sox32 in DFCcnpy1-MO+mRFP (E) or DFCcnpy1-MO + Cdh1 (F) embryos at 80% epiboly. (Scale bar: 200 μm.) (G) Percentage of broken-up DFC phenotype in mRFP-overexpressing (n = 82) or Cdh1-overexpressing (n = 103) DFCcnpy1-MO embryos. Overexpression of Cdh1 rescued the broken-up DFC phenotype in DFCcnpy1-MO embryos (60%; P < 0.05). (H) Diagram illustrating the FGF-dependent cell–cell communication control mechanisms of the forerunner cell cluster during early development. The model depicts the activation of intracellular FGF signaling via binding of Fgf8 ligands and Fgfr1 on the cell surface of two adjacent DFCs (blue ovals). The amplified FGF signal, through Cnpy1-mediated maturation of Fgfr1 within DFCs, subsequently activates the expression of tbx16 and cdh1 to organize forerunner cells as a cluster.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cdh1 WT control Shield forerunner cell group ISH
WT + MO1-cnpy1 standard conditions Shield forerunner cell group ISH
sox32 WT + MO1-cnpy1 standard conditions 75%-epiboly forerunner cell group ISH
tbx16 WT control Shield forerunner cell group ISH
WT + MO1-cnpy1 standard conditions Shield forerunner cell group ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cnpy1 standard conditions Shield cell-cell adhesion mediated by cadherin decreased process quality, abnormal
Shield forerunner cell group distributed, abnormal
75%-epiboly cell-cell adhesion mediated by cadherin decreased process quality, abnormal
75%-epiboly forerunner cell group distributed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA