ZFIN ID: ZDB-FIG-110719-5
Sorokina et al., 2011 - MIP/Aquaporin 0 Represents a Direct Transcriptional Target of PITX3 in the Developing Lens. PLoS One   6(6):e21122 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Protruding-mouth

Fig. 7

Analysis of mip1 expression in pitx3-mo and control embryos via in situ hybridization and RT-PCR.

A, D, F-H. Normal mip1 expression in control-injected embryos at 29-, 34- and 48-hpf. B, C, E, I–K. Altered mip1 expression is observed in pitx3 morphants at 29-hpf with 64% of embryos demonstrating a complete absence of mip1 expression (B) and the remaining larvae showing markedly reduced mip1 expression (C and I). Reduced mip1 expression is also observed in 34- and 48-hpf embryos (E, J, K). Red arrows show sites of expected mip1 expression. Scale bars: A–E: 100 μM; F–L: 20 μM. L. Results of semi-quantitative RT-PCR showing reduced expression of mip1 in pitx3 morphants at early stages of development (red arrow).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
mipa WT control Prim-5 solid lens vesicle ISH
Prim-15 lens ISH
Long-pec lens ISH
WT standard conditions 20-25 somites Not Detected whole organism RTPCR
26+ somites whole organism RTPCR
Prim-5 whole organism RTPCR
Prim-15 whole organism RTPCR
Protruding-mouth whole organism RTPCR
WT + MO3-pitx3 standard conditions 20-25 somites Not Detected whole organism RTPCR
26+ somites Not Detected whole organism RTPCR
Prim-5 Not Detected solid lens vesicle ISH
Prim-5 whole organism RTPCR
Prim-15 lens ISH
Prim-15 whole organism RTPCR
Long-pec lens ISH
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One