ZFIN ID: ZDB-FIG-110719-3
Sorokina et al., 2011 - MIP/Aquaporin 0 Represents a Direct Transcriptional Target of PITX3 in the Developing Lens. PLoS One   6(6):e21122 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 5

Injection of pitx3 morpholino results in abnormal splicing of pitx3 transcript and small lens phenotype.

A. Schematic drawing of pitx3 gene; position of initiation codon (ATG) and RT-PCR primers (1F and 3R) are indicated, exons are numbered. B. Sequencing of the pitx3-mo transcript generated with primers located in the first and third pitx3 exons demonstrates absence of exon 2 in the resultant product. C. RT-PCR results obtained with pitx3 1F/3R primers using RNA extracted from embryos injected with control or pitx3 morpholino. Please note a strong decrease in normal 276-bp product (black arrowhead) and presence of abnormal 148-bp product (red arrowhead) in pitx3-mo samples; hpf- hours post fertilization of analyzed embryos. D–G. Morphological phenotypes of zebrafish pitx3-mo embryos. In comparison to control embryos, a smaller head can be observed in pitx3 morphants (72-hpf embryo is shown; D) as well as an obvious reduction in lens size (black arrow) at later stages (96-hpf embryos are shown; E–G). H and I. Expression of pitx3 in the developing lens in 24-hpf embryos. Please note robust expression in the lens vesicle (arrows) as demonstrated by both whole mount (H) and section (I) in situ.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pitx3 WT control Prim-5 solid lens vesicle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-pitx3 standard conditions Protruding-mouth head decreased size, abnormal
Day 4 lens decreased size, abnormal
Day 4 lens development in camera-type eye disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One